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Curran Laboratory

Summary:

The Curran laboratory studies brain development and pediatric brain tumors. The goal is to identify molecular changes and potential drug targets. Additional studies focus on the mechanism of action of anticancer drugs in tumor cells and cancer models.

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Organisms and Viruses

  • Crk(loxP/loxP) ( Mus musculus )

    The targeting construct contained the first exon of Crk, flanked by two loxP sites, and followed by a NeoTk and another loxP site. Linearized gene-targeting vector was electroporated into 129/SvEv mouse embryonic stem (ES) cells. ES clones were microinjected into mouse blastocysts. Mice were bred with C57BL/6 mice.

    This line can be used to generate tissue-specific Crk knockouts.

  • Crk(loxP/loxP); Nestin-Cre ( Mus musculus )

    Nestin-Cre transgenic mice from Jackson laboratory were bred with floxed Crk mice.

  • Crk(loxP/loxP);CrkL(loxP/loxP);Nestin-Cre ( Mus musculus )

    The CrkL floxed allele carries three loxP sites, since the NeoTK gene is present, whereas only two loxP sites, flanking the first exon, are present in the Crk floxed allele.

    Crk is completely absent and CrkL expression is dramatically reduced in the cerebellum. Crk is not expressed in the hippocampus and CrkL expression decreased dramatically. Some cells, including pyramidal neurons in the CA3 region of the hippocampus, retained mild CrkL expression.

  • CrkL(loxP/loxP) ( Mus musculus )

    129/SvEv mouse ES cells were electroporated with a linearized targeting construct that contained the first exon of CrkL, flanked by two loxP sites, followed by a NeoTK gene and another loxP site. C57BL/6 × 129 chimeras were obtained.

    This line can be used to generate tissue-specific CrkL knockouts.

  • CrkL(loxP/loxP); Nestin-Cre ( Mus musculus )

    Nestin-Cre transgenic mice from Jackson laboratory were bred with floxed CrkL mice.

  • Ne-reelin ( Mus musculus )

    Myc-tagged reelin under the control of the rat nestin intermediate-filament gene promoter was introduced into fertilized one-cell FvB mouse embryos by microinjection. Mice carrying ne-reelin express an additional source of reelin mRNA throughout the ventricular zone of the forebrain, midbrain, and cerebellum at E16.5.

    ne-reelin was present at levels of approximately 10% and 20% of the endogenous Reelin in two founder lines.

  • rl/rl ne-reelin ( Mus musculus )

    Myc-tagged reelin under the control of the rat nestin intermediate-filament gene promoter was introduced into fertilized one-cell FvB mouse embryos by microinjection. Ne-reelin mice (strain FvB) were bred to reeler mice (strain B6C3Fe) from the Jackson Laboratory to generate mice that were transgenic for ne-reelin and mutant for reelin.

    Mice carrying ne-reelin express an additional source of reelin mRNA throughout the ventricular zone of the forebrain, midbrain, and cerebellum at E16.5.


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Last updated: 2013-02-20T15:51:27.329-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016