This strain is generated from the backcross of C57BL/6.C-H-2bm1/ByBir-gusmps mice onto the C3H-HeOuJ strain.
MPS VII mice (C57BL/6.C-H-2bml/ByBir-gusmps/mps) are generated by inbreeding the carrier strain C57BL/6.C-H-2bml/ByBir-gusmps/+.
This colony has been maintained since 1990 when carrier mice were purchased from The Jackson Laboratory.
This line was generated by backcrossing FVB-Tg(GadGFP)45704Swn/J from The Jackson Laboratory with C57BL/6.C-H-2bm1/ByBir-gusmps.
This line is inbred.
These mice (first described by the group of Dr. William S. Sly) constitutively express high levels of human GUSB. The transgene is expressed on the MPS VII mouse background, so only human GUSB enzyme is produced.
"Male pronuclei of both F1 and F2 zygotes were microinjected with the EcoRV fragment of the human beta-glucuronidase cosmid clone. The F1 zygotes were derived from C57BL/6J x LT/Sv crosses, and the F2 zygotes were derived from an intercross of C57BL/6J x SJL/J F1 hybrids. The human GUSB was placed onto the MPSVII mouse genetic background by mating male mice heterozygous for the human transgene with female mice homozygous for the gusmps mutation. TgGUSB/+,gusmps/+ were then mated to offspring without the human transgene to generate Tg-GUSB/+,gusmps/gusmps."
"The transgene contains an estimated 2 to 4 tandem repeat copies of 1.6 kilobases (kb) of the 5′ flanking sequence, the entire structural gene, and 3.8 kb of the 3′ flanking sequence of the human genomic GUSB gene. Thus, the TG mice produce no murine GUSB protein but maintain a normal phenotype because they produce 10 to 20 times higher than normal levels of human GUSB. They have been interbred to homozygosity and are maintained as a recombinant inbred strain."