The mission of the Rodent Metabolic Phenotyping Core is to provide the necessary resources and expertise to allow investigators to perform state-of-the-art studies of metabolism in rodent models.
The Specific Aims of the RMPC are to:
Provide access to state-of-the-art resources and expertise for rodent metabolic studies. Rodents have served as important models for human diseases and our understanding of diabetes, obesity and other metabolic disorders has increased tremendously as a result of dietary and genetic manipulations in these animals. In many cases, the standard techniques for phenotyping rodents are not accessible to individual investigators because they require expert surgical skills and facilities for infusion and handling of radioactive isotopes (e.g., clamps) or prohibitively expensive equipment that requires training and expertise to maintain (e.g., indirect calorimetry). By providing these services, the RMPC reduces duplication of expensive equipment and personnel, and ultimately limits research costs. These goals are well aligned with the overall mission of the DRC to promote and facilitate research into diabetes and metabolism.
Provide individualized guidance and assistance for designing and implementing in vivo metabolic assays. A key function of the core is to make metabolic assays accessible to investigators who would otherwise not be able to perform such studies. In many cases, this involves providing guidance as to the selection of appropriate assays within the suite of services offered by the core, and we are happy to do this.
Promote interactions and synergy among cores of the DRC. The RMPC fosters close interactions with the Radioimmunoassay/Biomarkers, Islet Cell Biology, Transgenic and Chimeric Mouse, Functional Genomics, Viral Vector, and Metabolomics cores in order to optimize metabolic studies in rodent models.
Yin, Xiaoyan., MD
Role: Research Specialist C; Mouse Phenotyping, Physiology and Metabolism Core
"Recording an animal’s body temperature accurately has never been easier. With the IPTT-300, researchers can collect both identification and temperature data with one transponder."
For use at room temperature.
CLAMS is available for use at room, thermoneutral or cold temperatures. Feeding, energy expenditure, and locomotor activity (Optovarimax System) are monitored.
SubCue Dataloggers have a biocompatible surface which allows implantation for monitoring temperature. Dataloggers have a memory capacity of over 2000 temperature measurements.
The tail cuff method is used to measure blood pressure and heart rate.
Body composition analysis including Body fat, lean mass, body water:
* dual energy x-ray absorptiometry (DEXA): performed on anesthetized animals
* Nuclear Magnetic Resonance (NMR): performed on live, un-anesthetized animals
Acute and chronic metabolic monitoring utilizes a Comprehensive Laboratory Animal Monitoring System (CLAMS).
• 72 hr. energy expenditure, feeding, drinking, locomotor activity monitoring
• Treadmill exercise and 2 hour energy expenditure
• 3 hr. energy expenditure following adrenergic agonist stimulation (NE or CL)
Coordination assays are carried out by infrared locomotor activity monitoring and include include:
• beam balance
Researchers have access to a broad range of metabolic tests.
-- Glucose tolerance test (intraperitoneal)
-- Insulin tolerance test (intraperitoneal)
-- Pyruvate tolerance test (intraperitoneal)
-- In vivo insulin signaling (Bolus IV insulin injection + tissue harvesting)
Lipid metabolism assays include:
-- In vivo fatty acid uptake assay (R-2-Bromopalmitic acid [9, 10-3H])
-- In vivo triglyceride production rate
Costs for individual surgical services: Users are only charged for successful surgeries (animals healthy and functional, patent catheters):
Arterial or jugular catheterization
Arterial and jugular catheterization
-- Stable isotope infusions
-- Hyperinsulinemic-euglycemic clamp
-- Hyperinsulinemic-euglycemic clamp with [3-3H]
-- Hyperinsulinemic-euglycemic clamp with [3-3H] and [14C] deoxyglucose
-- Hyperglycemic clamp
-- Clinical Chemistry Analysis (IDEXX VetTEST Chemistry Analyzer)
-- Quantitative Enzymatic-Colorimetric Assays
-- Manual measurement of Body weight, food intake and/or supply of special diet/water
-- Tail Blood Collection
-- Retro-orbital injection
-- Cold tolerance test (22°C vs. 4°C) combined with core temperature or BAT temperature measurements
-- Continuous core body temperature measurements via Anipill capsule peritoneal implants
-- BAT temperature measurements via subcutaneous implants
-- FLIR Thermal Imaging (22°C vs. 4°C)