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Mouse Phenotyping, Physiology and Metabolism Core (Penn)

Director: Ahima, Rexford S., M.D., Ph.D.

Summary:

Core Aims:

To offer expertise and services for determining the metabolic and endocrine phenotpyes of mice with diabetes, obesity and related disorders.

To create a database of physiologic, endocrine and metabolic measurements in mice.

Affiliations:

People:

Resources:

Instruments

Services

  • Blood pressure and heart rate measurement ( Analysis service )

    The tail cuff method is used to measure blood pressure and heart rate.

  • Body composition analysis ( Analysis service )

    Body composition is analyzed using dual energy x-ray absorptiometry (DEXA) and magnetic resonance imaging (MRI). Grip strength testing is also available.

  • Brown adipose tissue temperature measurement ( Analysis service )

    Brown adipose tissue temperature is measured using an implantable programmable temperature transponder.

  • Cold tolerance test ( Analysis service )

    Testing includes cold tolerance test, room temperature vs. 4ºC, core temperature and BAT temperature measurements. Thermographic imaging is also available.

  • Comprehensive metabolic monitoring ( Material analysis service )

    Acute and chronic metabolic monitoring utilizes a Comprehensive Laboratory Animal Monitoring System (CLAMS).
    Services include:

    • Energy expenditure and activity monitoring
    • 24 hr. energy expenditure, feeding, drinking, activity monitoring and sleep epochs
    • Treadmill exercise and energy expenditure

  • Coordination testing ( Analysis service )

    Coordination assays are carried out by infrared locomotor activity monitoring and include include:

    • beam balance
    • rotarod

  • Gluocose metabolism assays ( Analysis service )

    Researchers have access to a broad range of metabolic tests.

    • Intraperitoneal glucose, insulin and pyruvate tolerance tests
    • Insulin clamp and tracer studies
    • Hyperglycemic clamp and insulin secretion
    • Serum insulin ELISA
    in vivo insulin signaling (Bolus IV insulin injection and tissue harvesting)

  • Lipid metabolism assays ( Analysis service )

    Lipid metabolism assays include:
    in vivo fatty acid oxidation (3H-oleic acid)
    in vivo triglyceride production rate

  • Other mouse phenotyping, physiology and metabolism services ( Support service )

    • Intracerebroventricular cannulation
    • Jugular vein catheterizaion
    • Euthanasia and terminal blood collection


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Last updated: 2016-05-11T15:20:50.652-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016