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Wen-Chao Song Laboratory

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Organisms and Viruses

  • C3(-/-) ( Mus musculus )

    C57BL/6-C3−/− (G6 backcross) from Dr. Rick Wetsel (University of Texas Health Sciences Center at Houston) were further backcrossed in-house. In the C3 gene: "A genomic fragment containing 2.3 kb of the 5' flanking region and the first 105 bp of exon 1 was replaced with a neomycin selection cassette."

    This strain is cryo-preserved.

  • C3aR KO ( Mus musculus )

    Rick Wetsel developed original strain.

  • C4(-/-) ( Mus musculus )

    C4 knockout (C4−/−) mice were from The Jackson Laboratory (Bar Harbor, ME). "A genomic fragment corresponding to 987 nucleotides of coding sequence (exons 23-29) was replaced with a neomycin selection cassette."

    This strain is cryo-preserved.

  • C5aR GFP-KI ( Mus musculus )

    "A loxP site was inserted upstream of exon 2. A cassette containing (5' to 3') an internal ribosomal entry site (IRES), EGFP, SV40 polyadenlyation sequence, FRT-flanked neo cassette and loxP site was inserted into the 3' UTR."

    This strain is cryo-preserved.

    Gene targeting in embryonic stem (ES) cells was performed according to standard protocols. Linearized targeting vector was transfected into ES cells (TL-1, a cell line derived from the 129/SvEv mouse strain). The neomycin and diphtheria toxin genes on the vector allowed for double selection of ES cells that underwent homologous recombination and incorporated GFP and loxP into the C5ar1 gene locus on one strand of their genomic DNA. Positive clones were expanded, confirmed in second-round screening, and microinjected into C57BL/6 mouse blastocysts before being transferred to pseudopregnant females. Male chimeras were mated subsequently with female C57BL/6 mice (The Jackson Laboratory) to achieve germline transmission.

    "GFP is expressed highly on Gr-1+CD11b+ cells in the blood, spleen and bone marrow, and moderately on CD11b+F4/80+ circulating leukocytes and elicited peritoneal macrophages. No GFP is detected on resting or activated T lymphocytes, nor on splenic myeloid or plasmacytoid dendritic cells."

  • C5aR(-/-) ( Mus musculus )

    A breeder pair of C5a receptor knockout (C5aR−/−) mice on the C57BL/6 background was provided by Dr. J. Lambris.

    In these mice, "A PGK-neomycin resistance cassette replaced the entire coding region of the gene."

  • C6 KO ( Mus musculus )

    Tod Merkel (NIH) developed original strain.

    This strain is cryo-preserved.

  • CD59(-/-) ( Mus musculus )

    "Exon 2, which encodes the initiator methionine and the N-terminal signal peptide, was replaced with a neo cassette."

  • CD59(-/-) ( Mus musculus )

    "Exon 2, which encodes the initiator methionine and the N-terminal signal peptide, was replaced with a neo cassette."

    Cryopreserved - no active mice

  • CD97 KO ( Mus musculus )

    Jackson laboratory developed original strain.

    Cryopreserved - no active mice

  • CRIg KO ( Mus musculus )

    Genentech developed original strain.

  • Crry flox/flox ( Mus musculus )

  • Crry(-/-)/C3(-/-) ( Mus musculus )

    These mice were a gift from Dr. Hector Molina. Insertion of a neomycin resistance cassette in exon 5 resulted in mice deficient in Crry. Heterozygous mice, obtained from gene targeting in embryonic stem cells and blastocyst injection, were crossed with C3-/- mice and subsequently intercrossed to generate Crry-/-C3-/- mice. Crry and C3 are absent from platelets.

  • DAF(-/-) ( Mus musculus )

    Mice were generated by gene targeting. "Exons 2 and 3, encoding the first and second short consensus repeats, were replaced with a neomycin selection cassette."

    This strain is cryo-preserved.

  • DAF(-/-) ( Mus musculus )

    Mice were generated by gene targeting. "Exons 2 and 3, encoding the first and second short consensus repeats, were replaced with a neomycin selection cassette."

    Cryopreserved - no active mice

  • DAF(-/-)/C3(-/-) ( Mus musculus )

    DAF-/- mice were crossed with C3-/- mice.

  • DAF(-/-)/CD59(-/-) ( Mus musculus )

    DAF-/- were cross-bred with CD59-/- mice.

  • DAF(-/-)/CD59(-/-) ( Mus musculus )

    DAF-/- were cross-bred with CD59-/- mice.

  • DAF(-/-)/CD59(-/-)/C3(-/-) ( Mus musculus )

    DAF-/-CD59-/- were crossed with C3-/- mice. The C3-/- line (G6 backcross) is from The Jackson Laboratory (Bar Harbor, ME). The C3−/− mouse was further backcrossed in-house to G11.

  • DAF(-/-);Crry(-/-);C3(-/-) ( Mus musculus )

    Crry-/-/C3−/− and DAF-/-/C3−/− mice were crossed.

  • Factor D KO ( Mus musculus )

    Yuanyuan Xu (John E. Volanakis) developed original strain.

    This strain is cryo-preserved.

  • fB(-/-) ( Mus musculus )

    Exons 3 through 7 of the complement factor B gene (Cfb) were replaced with a neomycin resistance cassette.

    This strain is cryo-preserved.

  • fH(m/m) ( Mus musculus )

    Two stop codons were introduced at the beginning of short consensus repeat 19 of the mouse fH gene by transfecting TL-1 embryonic stem cells with linearized targeting vector. Selected clones were used for blastocyst microinjection to produce chimeric mice. Mice were bred to homozygosity.

    This strain is cryo-preserved.

  • fH(m/m)/fP(-/-) ( Mus musculus )

    This line was created by crossing fHm/m and fP-/- mice.

    This strain is cryo-preserved.

  • fP(-/-) ( Mus musculus )

    "A targeting vector was constructed where a loxP site was inserted upstream of exon 3, and a FRT flanked neomycin resistance cassette followed by a loxP site was inserted downstream of exon 5. Upon recombination, the neomycin cassette inserted in the intended location but the upstream loxP site failed to recombine. Analysis of chimeric mice revealed that the insertion of the neomycin cassette was sufficient to prevent transcription of the targeted gene. Gene inactivation was confirmed by failure to detect the gene product in the sera of homozygotes by an immunodiffusion assay."

    This strain is cryo-preserved.

  • fP(fl/fl) ( Mus musculus )

    "A targeting vector was constructed where a loxP site was inserted upstream of exon 3, and a FRT flanked neomycin resistance cassette followed by a loxP site was inserted downstream of exon 5. Flp mediated recombination removed the neo cassette leaving exons 3 through 5 floxed."

    This strain is cryo-preserved.

  • fP(fl/fl);EIIa-Cre ( Mus musculus )

    fPfl/fl mice were crossed with Ella-Cre transgenic mice (The Jackson Laboratory), which express Cre in oocytes and is often used as a universal deleter of floxed genes. Exons 3-5 of Cfp gene were deleted.

  • fP(fl/fl);Lys-Cre ( Mus musculus )

    Cfpfl/fl mice were crossed with lysozyme-Cre (Lys-Cre) transgenic mice, which express the Cre recombinase specifically in myeloid lineage cells.

  • Human Properdin Tg ( Mus musculus )

  • KRN ( Mus musculus )

    "The sequences of the variable regions of the TCR alpha and beta chains of the R28 hybridoma with rearranged Va4Ja27 and Vb6Jb1.5 genomic DNA fragments were amplified and cloned into TCR expression cassettes. Transgene-derived clones are transcribed under the control of the natural TCR alpha and -beta promoter/enhancer elements. A single founder (KRN) in which TCRalpha and Tcrbeta transgenes were cointegrated was identified. These constructs together express a T cell receptor that recognizes the 41-61 peptide of bovine pancreas ribonuclease (RNase) in the context of Ak."

    Diane Mathis developed original strain.

    This strain is cryo-preserved (NIH).

  • Properdin Δ/Δ ( Mus musculus )

    This strain is cryo-preserved.

  • Sult1e1 KO ( Mus musculus )

    Cryopreserved - no active mice


Last updated: 2016-06-09T14:09:41.680-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016