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Small Animal Imaging Facility: Optical/Bioluminescence Sub-Core (Penn)

Director: Delikatny, Jim, Ph.D.

Summary:

The Optical/Bioluminescence Sub-Core of the SAIF provides the capability to perform cellular and molecular non-invasive in-vivo bioluminescence, near-infraredfluorescence and Cerenkov imaging.

The instrumentation allows sensitive, non-invasive molecular imaging for a variety of applications including detection and quantification of various bioluminescent or fluorescent reporter-expressing cells or tissues (in culture or in small animals).

The facility currently houses a Perkin Elmer IVIS Lumina II, two LI-COR Pearl Impulse Imagers and two Perkin Elmer IVIS Spectrums. The Facility offers assistance with experimental design, regulatory approval, troubleshooting, data management, analysis and display.

Affiliations:

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Instruments

  • IVIS Lumina II ( In vivo bioluminescence imaging system )

    • In-vitro Imaging: cultured cell imaging, tissue/organ imaging
    • Bioluminescence imaging
    • Fluorescence imaging - GFP, DsRed, Cy5.5, ICG

  • LI-COR Pearl Impulse ( Small-animal image acquisition device )

    • In vitro and in vivo fluorescence imaging
    • 700 nm, 800 nm channels - IRDye® 680, IRDye® 700DX, IRDye® 800RS, IRDye® 800CW, Alexa Fluor® 680, Alexa Fluor® 750, Cy5.5, Cy7
    • Simultaneous detection of 700 nm and 800 nm channels

  • Perkin Elmer IVIS Spectrum ( Small-animal image acquisition device )

    • Advanced in vitro and in vivo bioluminescence and fluorescence imaging system
    • 10 fluorescence excitation filters and 20 fluorescence emission filters
    • Spectral unmixing and 3D imaging capability

Services


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Last updated: 2016-04-28T11:50:27.250-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016