The Optical/Bioluminescence Sub-Core of the SAIF provides the capability to perform cellular and molecular non-invasive in-vivo bioluminescence, near-infraredfluorescence and Cerenkov imaging.
The instrumentation allows sensitive, non-invasive molecular imaging for a variety of applications including detection and quantification of various bioluminescent or fluorescent reporter-expressing cells or tissues (in culture or in small animals).
The facility currently houses a Perkin Elmer IVIS Lumina II, two LI-COR Pearl Impulse Imagers and two Perkin Elmer IVIS Spectrums. The Facility offers assistance with experimental design, regulatory approval, troubleshooting, data management, analysis and display.
Browning, Elizabeth A., Ph.D.
Role: Technical Director, Optical/Bioluminescence Sub-Core, Small Animal Imaging Facility
• In-vitro Imaging: cultured cell imaging, tissue/organ imaging
• Bioluminescence imaging
• Fluorescence imaging - GFP, DsRed, Cy5.5, ICG
• In vitro and in vivo fluorescence imaging
• 700 nm, 800 nm channels - IRDye® 680, IRDye® 700DX, IRDye® 800RS, IRDye® 800CW, Alexa Fluor® 680, Alexa Fluor® 750, Cy5.5, Cy7
• Simultaneous detection of 700 nm and 800 nm channels
• Advanced in vitro and in vivo bioluminescence and fluorescence imaging system
• 10 fluorescence excitation filters and 20 fluorescence emission filters
• Spectral unmixing and 3D imaging capability
Services include in vivo bioluminescence and fluorescence imaging.
The facility assists with rodent anesthesia, substrate injection, animal shaving, image acquisition, data quantification and analysis, experimental design and protocol planning.