The Optical/Bioluminescence Sub-Core of the SAIF provides the capability to perform cellular and molecular non-invasive in-vivo bioluminescence, near-infraredfluorescence and Cerenkov imaging.
The instrumentation allows sensitive, non-invasive molecular imaging for a variety of applications including detection and quantification of various bioluminescent or fluorescent reporter-expressing cells or tissues (in culture or in small animals).
The facility currently houses a Perkin Elmer IVIS Lumina II, two LI-COR Pearl Impulse Imagers and two Perkin Elmer IVIS Spectrums. The Facility offers assistance with experimental design, regulatory approval, troubleshooting, data management, analysis and display.
Role: Technical Director, Optical Bioluminescence Sub-Core
• In-vitro Imaging: cultured cell imaging, tissue/organ imaging
• Bioluminescence imaging
• Fluorescence imaging - GFP, DsRed, Cy5.5, ICG
• In vitro and in vivo fluorescence imaging
• 700 nm, 800 nm channels - IRDye® 680, IRDye® 700DX, IRDye® 800RS, IRDye® 800CW, Alexa Fluor® 680, Alexa Fluor® 750, Cy5.5, Cy7
• Simultaneous detection of 700 nm and 800 nm channels
• Advanced in vitro and in vivo bioluminescence and fluorescence imaging system
• 10 fluorescence excitation filters and 20 fluorescence emission filters
• Spectral unmixing and 3D imaging capability
Services include in vitro imaging of cell culture or tissue/organ.
Services include in vivo bioluminescence and fluorescence imaging.
The facility assists with rodent anesthesia, substrate injection, animal shaving, image acquisition, data quantification and analysis, experimental design and protocol planning.