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Radioimmunoassay and Biomarkers Core (Penn)

Director: Rickels, Michael R., M.D., M.S.

Summary:

"The assay service has proven essential for current research in the DRC and Penn community. The core used to focus mainly on rat and human insulin, glucagon and C-peptide to a diverse, high-volume and cost-effective service. Over 50 different diabetes and endocrinology-related markers can be assayed.

In conjunction with the Clinical and Translational Research Center (CTRC), we are now offering multiple analyte (multiplex) services. As part of a collaborative initiative, Dr. Collins initiated and established Luminex IS100 multiplex ELISA services. This system uses cell-sorting technology to measure multiple proteins simultaneously. This technology will be of great use for investigators studying transgenic mice, where sample volumes are low. In addition, the multiplex platform allows for screening of human cohorts in disease research particularly of small volume in repeated sampling protocols.

The objectives of the Radioimmunoassay and Biomarker Core includes:

• delivery of new services to existing and new investigators
• development of informatics infrastructure for efficient delivery of service
• engage proactively in outreach to the DRC/PENN research community to enhance efficiency, cost-sharing and increase users of Biomarker Core services."

The assay services listed are not an exhaustive list but rather a list of assay services we have provided in the past. Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit and provide the assay service.

Affiliations:

People:

Resources:

Instruments

  • Luminex LX100 ( Bead array reader )

    "The Luminex 100/200 System is a flexible analyzer based on the principles of flow cytometry. The system enables you to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small sample volumes. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid assays, receptor-ligand assays, immunoassays and enzymatic assays."

    The Luminex LX100 can use both polystyrene and magnetic bead kits.

  • Perkin Elmer 1470 Wizard gamma counter ( Gamma counter )

  • SpectraMax M2 plate reader ( Microplate reader )

    "The SpectraMax® M2 & M2e Multi-Mode Microplate Readers provide two detection modes in one platform and has the the added functionality of bottom read fluorescence for cell-based assays.

    • UV-Visible Absorbance (Abs)
    • Fluorescence Intensity (FI- Top Read)

    The SpectraMax M2 and M2e Microplate Readers are the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates.

    Using the patented, PathCheck® PathLength Measurement Technology, the SpectraMax M2 & M2e Microplate Readers transform each well in a microplate to a fixed optical pathlength cuvette, and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation.

    For fluorescence intensity assays, the SpectraMax M2 and M2e Microplate Readers optical design provides the highest level of flexibility. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays (M2e only). Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1 nm. Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements, allowing for fast setup of FRET assays.

    The SpectraMax M2 and M2e Microplate Readers are supplied with SoftMax® Pro Data Acquisition & Analysis Software, Molecular Devices' industry leading all-in-one software. Among the various ready-to-read protocols, users will find pre-written SpectraDrop™ Plate protocols enabling Micro Volume applications measurement. Additionally, the SpectraMax M2 and M2e Microplate Readers can be seamlessly integrated with the StakMax® Microplate Handling Stacker through SoftMax Pro Software."

Services

  • Adiponectin assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Adrenocorticotropic hormone assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Androstenedoione assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • C-peptide assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Corticosterone assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Cortisol assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Cyclic AMP assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Dehydroepiandrosterone assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Dihydrotestosterone assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Estrogen assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Estrone assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Follicle stimulating hormone assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Free triiodothyronine assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Gamma counter access ( Access service )

    Users have access to the Perkin Elmer 1470 gamma counter.

  • Glucagon assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Glucagon-like peptide 1 assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Growth hormone assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Insulin assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Insulin-like growth factor I assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Leptin assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Luminex multiplex assays ( Material analysis service )

    Luminex IS100 multiplex ELISA services use both polystyrene (non-magnetic) and magnetic bead based kits. Kits are available for human, canine, mouse, rat, non-human primate, pig, horse, and monkey.

    "This system uses cell-sorting technology to measure multiple proteins simultaneously. This technology will be of great use for investigators studying transgenic mice, where sample volumes are low. In addition, the multiplex platform allows for screening of human cohorts in disease research particularly of small volume in repeated sampling protocols."

    Please contact the facility for assistance in choosing appropriate kits.

  • Luteinizing hormone assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Monkey C-peptide assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Monkey insulin assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Oxytocin assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Pancreatic peptide assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Progesterone assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Proinsulin assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Prolactin assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Resistin assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Salivary cortisol assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Salivary estradiol assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Sex hormone binding globulin assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • SpectraMax M2 plate reader access ( Access service )

    Users have access to the plate reader for UV-visible absorbance and fluorescence intensity assays. Data can be transferred from the plate reader using a flash drive.

  • Thyroid stimulating hormone assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Thyroxine assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

  • Total testosterone assay ( Material analysis service )

    Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit.

Software

  • StatLIA ( Software )

    "StatLIA software for immunoassay and bioassay analysis uses the most advanced computations available in commercial software. StatLIA's industry leading TrueFit 5 and 4 parameter logistic curve fitting models provide superior accuracy for potency, quantitative (ELISA, FIA, RIA, etc.) and other assay technologies. The software's SmartQC analysis pools your previously run assays to compute accurate weighting and make statistical comparisons between the pooled and current assay. This comparative analysis provides powerful metrics on the performance of the assay's responses, precision, curve fits, concentrations, relative potency, and more that can only be computed by pooling previous assays."


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Last updated: 2016-05-16T12:58:23.360-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016