Mission and Goals:
The mission of the Metabolomic Core at CHOP (MC@CHOP) is to provide analytical services to advance understanding of metabolism in health and disease states. Our state-of-the-art research facility allow investigators to carefully study the relationship between metabolome, fluxome and disease states such as cancer, diabetes, inborn errors of metabolism, metabolic syndrome, urea cycle defects, traumatic brain injury, drug addiction, sleep disorder, etc.
Furthermore, MC@CHOP provides the analytical and theoretical wherewithal to investigate the impact of drugs on metabolism as well as the potential benefits and risks of a given drug treatment.
Role: Senior Research Associate and Metabolomic Core Coordinator
The MC@CHOP offers a variety of analytical techniques to quantitate stable isotope enrichment (13C, 2H, 15N) and to perform metabolic profiling. Our analytical repertoire includes the following:
Stable Isotope Analysis, including:
-- Stable isotope enrichment (13C, 15N, 2H, 18O) in glucose, amino acids, NH3, urea, tricarboxylic acid cycle intermediates, fatty acids and other intermediates of metabolism
-- 13CO2 enrichment in blood and/or expired air for studies of oxidative metabolism
-- Doubly labelled water (2H2O/H218O ratio) for studies of metabolic rate in a freely moving organism
-- Measurement - both in vivo and in vitro - of flux through metabolic pathways, such as glycolysis, the tricarboxylic acid cycle, fatty acid oxidation, the urea cycle, protein synthesis/degradation.
-- Metabolomics profiling including, measurements of amino acids, organic acids, NAD+, NADH, NADP+, NADPH, adenine nucleotides (AMP, ADP, ATP), Pi, triglycerides, free fatty acids, glucose, putrescine, pyridoxines, pyridoxal, protein, etc.
-- Carnitine / Acylcarnitines levels and profiling both in blood and tissue extracts
-- Measurement of neurotransmitters and neuromodulator levels and profiling
-- Measurement of D- or L- forms of amino acids and their levels both in physiological fluid and tissue extracts.
-- Measurement of selected drug and/or drug metabolites as well as determining the impact of a given drug on metabolome and fluxome.
-- Measurement of signaling compounds such as, cAMP in various tissues.