Our research focuses on developing mouse models of stress sensitivity related to neurodevelopmental and neuropsychiatric disease. We utilize genetic and prenatal manipulations to elucidate mechanisms contributing to disease predisposition.
We have focused on utilizing approaches that range from fetal antecedents in programming of long-term disease risk to genetic targeting of cell type specific knockout mice.
We have focused on developing models of disease including affective disorders and obesity utilizing approaches that range from fetal antecedents, involved in programming of long-term disease risk, to genetic targeting of cell type specific knockouts.
We have initiated multiple lines of investigation that will provide insight into the timing and sex specificity of early life events promoting disease susceptibility, the maturation of central pathways during key periods of development, and the epigenetic mechanisms involved in long-term effects following stress exposure.
"CRF2−/− mice were generated in-house on a mixed C57BL/6:129J background as described." A targeting vector was constructed "in which the portion of Crhr2 encoding one-half of the fifth transmembrane domain through the end of the seventh transmembrane domain was replaced with a neomycin-resistant gene cassette". Targeted ES cells were injected into C57BL/6 blastocysts to generate chimaeric mice.