"A promoterless neomycin resistance gene was inserted in frame into exon 2. RNase protection assays on RNA derived from liver of homozyogous mice demonstrated that no detectable alpha or delta isoform transcript was produced from this allele; however, a beta isoform transcript is upregulated. Western blot analysis on liver extracts from homozygous mice confirmed that no alpha or delta isoform of the encoded protein was produced."
"Mice containing the Creb1 gene with exon 10/11 flanked by loxP sites were maintained on a C57BL/6 background."
"Mice containing the Creb1 gene with exon 10/11 flanked by loxP sites were maintained on a C57BL/6 background."
"The construction of the A112G allele was accomplished by using a bacterial artificial chromosome (BAC) containing the entire Oprm1 locus derived from C57BL/6 mouse DNA. The region containing exon 1 and flanking introns was used as the template for site-directed mutagenesis.
The polymorphism in exon 1 was constructed by changing the
adenosine (A) nucleotide at position 112 of the mouse, which
corresponds to position 118 in the human sequence, to a
guanosine (G). A second mutation (T108C) was introduced."