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Research Viral Vector Core (CHOP)

Summary:

The Research Viral Vector Core (RVC) provides premium GLP recombinant Adeno-Associated Viral Vectors and Lentiviral Vectors for use in basic research and preclinical studies. A part of the Center for Cellular and Molecular Therapeutics at the Children's Hospital of Philadelphia, the RVC is dedicated to manufacturing top of the line vectors utilizing a fine tuned downstream process recognized internationally in industrial applications and academia. Capable of providing custom vector constructs at a variety of scales, The Research Vector Core offers state of the art technology and support for investigators interested in conducting viral based gene transfer.

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Services

  • Proviral Amplification and Cloning ( Support service )

    Upon request, proviral plasmids can be amplified. Additionally, targets can be cloned into a proviral backbone.

  • Quality Control ( Support service )

    Additional vector quality control services are available upon request. The core offers purity assessments, genomic, particle, and infectious titering, as well as mycoplasma, endotoxin, and bioburden assays.

  • Recombinant Adeno-Associated Viral Vectors ( Material production service )

    With access to a wide range of host organisms, long-term expression in vivo, and low immunogenicity, recombinant Adeno-Associated viral vectors (rAAV) offer a robust and proven method of gene delivery. rAAV is a single stranded DNA parvovirus that infects both dividing and non-dividing cells with varying tissue tropisms and transduction efficiencies. It is highly popular in both the pre-clinical and clinical settings, capable of producing high titers for transgenes at maximum cassette limits of 4.7kb for single stranded vectors, and 2.2kb for self-complementary vectors. Our facility is equipped to manufacture rAAV at large scales with a production capacity of 2.0 x 1012 - 3.0 x 1015 viral genomes per week and can purify and concentrate vectors up to 1-5 x 1013vg/ml. Optimized for enhanced transduction efficiency and purified via CsCl gradient centrifugation, our final product contains over 99% packed capsid to ensure the highest effective potency in your application.

  • Recombinant Lentiviral Vectors ( Material production service )

    Recombinant Lentiviral vectors (rLV) constitute a powerful gene delivery system capable of reliably transducing both dividing and non-dividing cells. rLV integration into the host genome produces long-term, stable expression of a gene of interest of up to 9.5kb and can be pseudotyped with a wide variety of viral envelope glycoproteins to broaden desired tropism. The RVC produces 3rd generation Self-Inactivating (SIN) Lentiviral vectors minimizing the risk of replication-competent virus and offers both crude supernatant and highly purified vector concentrate for your specific research and pre-clinical requirements.


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Last updated: 2019-10-10T14:11:20.627-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016