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High Throughput Sequencing Core (CHOP)

Summary:

The Children's Hospital of Philadelphia and the Beijing Genomics Institute (BGI) have formed a collaborative genome center entitled BGI@CHOP. Together, these elements form The High-throughput Sequencing (HTS) Core with increased capacity, expertise and analytical resources for conducting next-generation sequencing studies. The HTS Core will provide automated library construction and high-quality, high-throughput sequencing services for whole genome and whole exome samples.

Affiliations:

People:

    Resources:

    Instruments

    Services

    • 100 X 100 paired end sequencing ( Material analysis service )

      100 X 100 paired end sequencing is carried out with a user premade library.

      "FASTQ data files supplied as part of standard service. Additional analysis available upon request."

    • ChIP-Seq ( Material analysis service )

      • 20 million reads
      • SE 50bp sequencing

      "FASTQ data files supplied as part of standard service. Additional analysis available upon request."

    • RNA-seq ( Material analysis service )

      • 40 million reads
      • SE 50 bp sequencing

      "FASTQ data files supplied as part of standard service. Additional analysis available upon request."

    • Small RNA Seq ( Material analysis service )

      Small RNA Seq with standard report files
      • 30 million reads
      • SE 50bp sequencing

      "FASTQ data files supplied as part of standard service. Additional analysis available upon request."

    • Transcriptome sequencing ( Material analysis service )

      • 60 million reads
      • PE 100 bp sequencing

      "FASTQ data files supplied as part of standard service. Additional analysis available upon request."

    • Whole exome sequencing ( Material analysis service )

      Human (Agilent V4 51Mb Exome kit) or mouse exome (Agilent Mouse Exome kit) sequencing with standard report files. Several coverages are available: 30X, 50X, 100X, 150X or 250X.

    • Whole genome sequencing ( Material analysis service )

      Human whole genome sequencing carried with short insert library (≤ 500 bp) with gel sizing. Standard report files are delivered. Several coverages are available: 30X, 60X, or 100X.


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    Last updated: 2016-03-11T12:53:12.242-05:00

    Copyright © 2016 by the President and Fellows of Harvard College
    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016