The Stem Cell core provides expertise and quality-control reagents for the culture and differentiation of human embryonic stem cells (ESCs) and human induced pluripotent stem cells (iPSCs) to the CHOP and University of Pennsylvania academic communities. The facility maintains five of the new NIH-approved human ESC lines and several human iPSC lines. Stringent protocols are established to:
1. Propagate human ESC and iPSC lines, monitor for maintenance of the pluripotent state and distribute these lines to investigators.
2. Differentiate hESCs/iPSCs into mesoderm, endoderm and their derivative tissues under controlled, serum-free conditions with defined cytokines.
3. Generate iPSCs from human subjects.
French, Deborah L., PhD
Role: Research Associate Professor of Pathology and Laboratory Medicine, Department of Pathology and Laboratory Medicine, Perelman School of Medicine