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Mouse Cardiovascular Physiology and Microsurgery Core (Penn)

Director: Wang, Tao, Ph.D.

Summary:

The Mouse Cardiovascular Physiology Core Facility, established in April 2006, is under the leadership of Dr. Tao Wang. Our goal is to aid investigators with the creation of mouse models related to cardiovascular diseases. We provide comprehensive assessments of cardiovascular structure and function using high-resolution echocardiographic imaging tools, which provides a unique and valuable research opportunity for translational research from animal studies to human applications. We deliver on-demand services that reveal important cardiovascular, structural, and functional aspects of mouse models in an efficient and cost-effective manner.

We offer a variety of surgical procedures, mouse models of cardiovascular disease, invasive hemodynamical measurements of pressure and ventricle function, high-resolution ultrasound imaging, and ECG/BP telemetry services for the comprehensive evaluation of cardiovascular phenotypes of transgenic and knockout mice. We perform surgical procedures that may be difficult or time-consuming for investigators without the proper expertise, experience, and/or equipment. Furthermore, we provide surgical training for various mouse models and data analysis for physiological assessment of cardiovascular function.

Affiliations:

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Instruments

Services

  • Cardiovascular-related study support services ( Support service )

    "The Mouse Cardiovascular Physiology Core provides services to assess cardiovascular function in mouse models. We will work with investigators to design and implement the study along with any associated grant applications, animal protocol submissions and manuscript preparation. While the central focus of the facility is cardiovascular research, the techniques employed are often useful to investigators in other fields.

    Our Core staff will work with you to assess your needs and provide the necessary technical training and scientific assistance in animal protocol preparation. Investigators will be responsible for the costs incurred for their projects and prior animal protocol approval by Penn IACUC."

    Please contact the core director to determine the service fee.

  • Echocardiography (2-D, M-mode, PW Doppler, Tissue Doppler) for comprehensive evaluating cardiac structure and function ( Analysis service )

    • Prenatal mouse embryo ultrasound for developmental studies
    • Ultrasound assessment of cardiac function in adult mice
    • Left Ventricle Function
        - Systolic and Diastolic Analysis
        - Quantify cardiovascular function (ejection fraction, fractional shortening)
    • Right Ventricle Function
        - Wall thickness, FS%
    • Vessels Assessment
        - Visualize vessel wall movement and vascular pathologies including atherosclerosis
        - Assess flow through vessels and valves using Pulse Wave Doppler
    • Myocardial Wall Motion
        - Quantify myocardial mechanics using Strain as an early biomarker for cardiovascular dysfunction
    • Myocardial perfusion

    Please contact the core director to determine the service fee.

  • Hemodynamic assessments of cardiovascular function ( Analysis service )

    • Invasive blood pressure (BP) and pressure gradient measurement
    • Left ventricle (LV) catheterization and LV pressure measure assessment
    • Left ventricle pressure-volume loop assessment
    • Right ventricle (RV) catheterization and RV pressure measurement
    • Direct pulmonary pressure measurement (open chest)

  • Surgical procedures and animal model services ( Material processing service )

    • Myocardial ischemia/reperfusion (I/R) injury model
    • Permanent myocardial infarction model by LAD ligation model
    • Wire-induced femoral or carotid artery injury model
    • Transverse aorta constriction TAC (aka. aorta banding) model
    • Minipump implantation and drug-induced cardiac hypertrophy and fibrosis model
    • Pneumonectomy (left lobe) for lung stem cell research
    • Acute myocardial injury in the neonatal mice (Day 0-7 after birth, P0-P7) via Cardiac apical resection or left anterior descending coronary artery
    • ECG telemetry transmitter implantation
    • BP telemetry transmitter implantation
    • Minor surgery (IV injection, blood sample collection, organ harvesting, etc.)

  • Technical training in mouse microsurgery, analytic training in echocardiographic imaging and hemodynamic data analysis ( Training service )

    Please contact the core director to determine the service fee.

  • Telemetry: ambulatory ECG or arterial blood pressure recording and data analysis with Data Science International (DSI) telemetry unit ( Analysis service )

    • Telemetry device (ECG and/or BP transducer) implantation
    • ECG recording
    • BP recording

    Please contact the core director to determine the service fee.

  • Treadmill exercise tolerance test ( Analysis service )

    Please contact the core director to determine the service fee.


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Last updated: 2016-05-11T15:10:24.869-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016