eagle-i University of PennsylvaniaUniversity of Pennsylvania
See it in Search

Mouse Cardiovascular Phenotyping Core (Penn)

Summary:

The Mouse Cardiovascular Physiology Core provides services to assess cardiovascular function in mouse models and to provide validated surgical models of heart and vascular disease in mice to assess genetic or therapeutic interventions. We will work with investigators to design and implement the study along with any associated grant applications, animal protocol submissions and manuscript preparation. While the central focus of the facility is cardiovascular research, the techniques employed are often useful to investigators in other fields. Our Core staff will work with you to assess your needs and provide the necessary technical training and scientific assistance in animal protocol preparation. The Core runs on a fee-for-service model. Accordingly, investigators will be responsible for the costs incurred for their projects and prior animal protocol approval by Penn IACUC.

Affiliations:

People:

    Resources:

    Instruments

    Services

    • Cardiovascular-related study support services ( Support service )

      "The Mouse Cardiovascular Physiology Core provides services to assess cardiovascular function in mouse models. We will work with investigators to design and implement the study along with any associated grant applications, animal protocol submissions and manuscript preparation. While the central focus of the facility is cardiovascular research, the techniques employed are often useful to investigators in other fields.

      Our Core staff will work with you to assess your needs and provide the necessary technical training and scientific assistance in animal protocol preparation. Investigators will be responsible for the costs incurred for their projects and prior animal protocol approval by Penn IACUC."

    • Echocardiography (2-D, M-mode, PW Doppler, Tissue Doppler) for comprehensive evaluating cardiac structure and function ( Analysis service )

      • Ultrasound assessment of cardiac function in adult mice
      • Left Ventricle Function
          - Systolic and Diastolic Analysis
          - Quantify cardiovascular function (ejection fraction, fractional shortening)
      • Right Ventricle Function
          - Wall thickness, FS%
      • Vessels Assessment
          - Assess flow through vessels and valves using Pulse Wave Doppler
      • Myocardial Wall Motion

    • Hemodynamic assessments of cardiovascular function ( Analysis service )

      • Invasive blood pressure (BP) and pressure gradient measurement
      • Left ventricle (LV) catheterization and LV pressure measure assessment
      • Left ventricle pressure-volume loop assessment
      • Right ventricle (RV) catheterization and RV pressure measurement

    • Surgical procedures and animal model services ( Material processing service )

      • Myocardial ischemia/reperfusion (I/R) injury model
      • Permanent myocardial infarction model by LAD ligation model
      • Transverse aorta constriction TAC (aka. aorta banding) model
      • Combined transverse aortic constriction (TAC)/myocardial infarction (MI)
      • Minipump implantation and drug-induced cardiac hypertrophy and fibrosis model
      • Minor surgery (IV injection, blood sample collection, organ harvesting, etc.)


    Web Links:

    Last updated: 2020-01-10T09:59:04.382-05:00

    Copyright © 2016 by the President and Fellows of Harvard College
    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016