The Parmacek laboratory has a longstanding interest in examining the molecular programs that regulate vascular smooth muscle cell and cardiac myocyte development and differentiation. Dr. Parmacek cloned and characterized members of the GATA-4/5/6 family of zinc finger transcription factors and used gene targeting techniques in mice to elucidate the distinct functions of GATA-4 and GATA-6 in the cardiovascular system. The Parmacek laboratory also used transgenic techniques to show that an SRF-dependent transcriptional program regulates vascular SMC differentiation.
More recently, the Parmacek Lab reported that myocardin is a critical SRF cofactor that regulates vascular smooth muscle cell differentiation and modulation of smooth muscle cell phenotype. These studies are relevant to understanding the molecular basis of angiogenesis and the pathophysiology of vascular proliferative syndromes including atherosclerosis.
In collaboration with other Penn cardiologists, Dr. Parmacek performs translational research studies focusing on stem cells and other novel agents that may be used to treat cardiovascular disease.
Role: Parmacek Laboratory Manager
"A loxP site was inserted upstream of exon 8 and a neo cassette and loxP site were inserted downstream of exon 8 via homologous recombination. Cre mediated recombination deleted the neo cassette and exon 8."
"A neo cassette with a 3' loxP site was inserted into intron 8 and an additional loxP site was inserted into intron 7."