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Microbial Culture and Metabolomics Core (Penn/CHOP)

Directors: Friedman, Elliot, PhD; Wu, Gary, MD

Summary:

The Microbial Culture and Metabolomics Core features facilities and equipment for the aerobic and anaerobic culture of microbial species in both batch and continuous systems as well as services for targeted metabolomics. The core offers training and usage for all of these equipment as well as consultation towards experimental design and method development of microbial culture studies. Additionally, the core offers anaerobic culture services; working with the researcher, the core will purchase, receive, and revive strains from commercial culture collections (i.e., ATCC, DSMZ). The core will prepare glycerol stocks, liquid cultures, or gavage-ready suspensions for inoculation of animals with pure or define-mixed microbial communities.

Affiliations:

People:

Resources:

Instruments

  • Anaerobic chamber ( Environmental chamber )

    Type B anaerobic chamber for anaerobic microbial culture

    Anaerobic chamber

  • Anaerobic degassing station ( Vacuum degasser )

    Custom-built

  • Eppendorf BioFlo 320 ( Fermentor )

    "the core features an automated bioreactor (BioFlo 320, Eppendorf, Hauppauge, NY), which is capable of maintaining pH and dissolved oxygen levels at a given setpoint. This system can be operated in batch, fed-batch, or continuous modes and is compatible with the Eppendorf BioBlu Single-Use Vessels, which allows for operation of volumes between 0.32 – 40 L"

    Eppendorf BioFlo 320

  • PMOD 500 laser phosphorimeter ( Fluorescence and luminescence assay detection system )

    "The core also contains a laser phosphorimeter (PMOD 500, Oxygen Enterprises, LLC., Philadelphia, PA) for phosphorescent measurement of oxygen using OxyPhor G4."

  • Waters Acquity UPLC system with QDa single-quadrupole mass detector ( High performance liquid chromatography instrument )

    "This system is configured for bile acid, short chain fatty acid, and amino acid analysis using an autosampler with a capacity of 192 samples. Analyses can be performed in both plasma and fecal samples."

    Waters Alliance HPLC single-quadrupole MS

Services

  • Anaerobic culture services ( Material production service )

    "Additionally, the core offers anaerobic culture services; working with the researcher, the core will purchase, receive, and revive strains from commercial culture collections (i.e., ATCC, DSMZ). The core will prepare glycerol stocks, liquid cultures, or gavage-ready suspensions for inoculation of animals with pure or define-mixed microbial communities."

  • Microbial culture training service ( Training service )

    "The core offers training and usage for all of these equipment as well as consultation towards experimental design and method development of microbial culture studies."

  • Targeted metabolite analysis ( Material analysis service )

    "The metabolomics branch of the core features a Waters Acquity uPLC System with a QDa single quadropole mass detector. This system is configured for three classes of small molecules (i.e., bile acids, short chain fatty acids, and amino acids) using an autosampler with a capacity of 192 samples. The core will process, extract, and analyze your samples; we are currently accepting fecal (human and murine) samples for analysis, and are in the process of developing methods for measuring compounds in plasma and culture broth.

    We require at least 25 mg of fecal material per class (i.e., amino acids, SCFAs, bile acids) or at least 75 mg for a complete panel. Plasma and culture medium assays are currently under development."

  • Untargeted (discovery) metabolomics ( Material analysis service )

    "A 5% discount is offered on Metabolon services obtained through the core.

    Discovery metabolomics is a revolutionary technology for obtaining a comprehensive picture of the metabolome and mapping metabolites to their key biochemical pathways. It helps answer a wide variety of research questions and gives crucial insight into the function of genes, the basis of disease, and how treatments work. Discovery metabolomics also identifies the associated biomarkers, making it an attractive tool for investigators across multiple applications.

    For more information on Metabolon, please visit their website at: http://www.metabolon.com/"


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Last updated: 2016-05-11T14:59:51.232-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016