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Penn Vector Core- Gene Therapy Program

Directors: Johnston, Julie., PhD; Sandhu, Arbans K., PhD

Summary:

The Penn Vector Core is a full-service viral vector core facility located on the University of Pennsylvania campus. With over a decade of experience in the production of viral-based vectors, the Core has become an important technological resource for investigators, both within and external to Penn, interested in the use of viral-based vectors for gene transfer. The main objective of the Core is to provide investigators access to state-of-the-art vector technology for preclinical studies and other basic research applications. Such studies, utilizing carefully designed viral vectors, can provide information critical to the understanding of gene function and the development of therapeutic vectors. The Penn Vector Core specializes in the provision of novel AAV serotype vectors, and has the greatest experience in producing novel serotype vectors developed at Penn. AAV 1, 7, 8, 9, and rh10 were originally isolated at Penn in the laboratory of Dr. James M. Wilson, and first made available to investigators through the Penn Vector Core. Due to its close proximity to the Wilson laboratory, the Penn Vector Core is able to rapidly assimilate new vector technologies and make them available to its users. The Core offers a variety of novel serotype AAV vectors, and additional vectors currently under development will be distributed through the Penn Vector Core. All of the vectors generated by the Penn Vector Core are distributed under material transfer agreements (MTA) to academic, government, and non-profit institutions.

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Services

  • Penn Vector Core ( Material production service )

    Penn Vector Core standard services
    1) Four AAV production scales to match your vector needs
    2) ddPCR titration and Endotoxin assay on all preps
    3) Consultation and advice in the design of custom vectors

    Additional services that can be added to your AAV vector prep
    1) Plasmid cloning services
    2) Plasmid amplification and characterization
    3) SDS-PAGE purity
    4) Endotoxin assay
    5) ddPCR titration
    6) TCID50 infectious titer determination


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Last updated: 2019-12-03T15:31:29.412-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016