eagle-i University of PennsylvaniaUniversity of Pennsylvania
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Live Cell Imaging Core

Directors: Boesze-Battaglia, Kathleen., PhD; Dhingra, Anu., PhD

Summary:

The Penn Dental Medicine Live Cell Imaging Core features a Nikon A1R Confocal Microscope, that includes the following specifications and applications.
-- Transmitted light, DIC and epifluorescence (Dapi, FITC, TRITC filters)
-- Objectives: 20x Dry, 40x Dry, 60x water, 100x oil
-- Fully automated XYZ scanning stage — Piezo Z stage insert for high speed image capture when using resonance scanner; Perfect Focus System, allowing for accurate stage reposition over timed series events.
-- Environmental chamber — Adjustable CO2 percentage and temperature with constant humidity; Accommodates 35 mm glass bottom culture dishes, 30×60mm chamber slides and 25×75mm glass slides.
-- Scan Heads for Image capture — Galvo (up to 4 fps (512×512); Resonance 30 fps (512×512 up tp 230 fps (512×64)
-- Stage — Fully automated XYZ scanning; Piezo Z stage insert for high speed image capture with resonance scanner; Perfect Focus System, allowing accurate stage reposition over a time series.
-- 4 Laser lines with filter configuration
-- Detectors — 4- Dedicated PMT for laser lines; 1-DIC PMT; DUS Spectral detector- 32 channels of detection over the 400nm-750mn. Spectral resolution adjusted to 2.5nm, 6nm and 10nm channel width.
-- Image Analysis — Nikon NIS Elements software; Additional Elements Software Workstation
-- Examples of Applications — Live cell (GFP translocation, Phagocytic uptake, LPS induced toxicity, Spectral profiling of inclusion bodies, Bimolecular fluorescence complementation) and Fixed (4-color imaging, Whole retina mounts, Protein co-localization, 3D targeted protein expression, FRET)

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Last updated: 2019-12-13T09:35:34.432-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016