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Core Laboratories at University of Pennsylvania

This is a summary list of all core laboratories at University of Pennsylvania . The list includes links to more detailed information, which may also be found using the eagle-i search app.

Acute Care Biobanking Core (Penn/CHOP)

Summary:

The goals of the Acute Care Biobanking Core are to encourage and facilitate microbiome-focused research in the pathogenesis, diagnosis and treatment of patients with critical illness. Many patients who are critically ill are subject to processes and complications with microbially-driven or infectious mechanisms. The Core will assist in research by providing de-identified samples with linked clinical metadata to support research in this area.



AddLab (Penn)

Summary:

Located on the first floor of the Towne engineering building, the AddLab houses the mechanical engineering department's 3D printers and post-processing equipment. The lab is generally staffed by student additive manufacturing assistants who are available to consult with members of the university on their 3D printing needs. The lab is restricted to approved workers who print and process all of the parts. If your interested in having anything 3D printed, please see our 3D printing page.



Advanced Optical and Force Microscopy Facility (Penn)

Summary:

The PMI has assembled a unique combination of microscopes for single-molecule-imaging and single-molecule-force measurements. Secured through funding from the NSF, NIH, NIST, University Research Foundation and PSOM, these state-of-the-art instruments are being used to address important biological questions, using purified macromolecules and in live cells. The instruments are only the starting point, as PMI investigators develop novel imaging technologies that will greatly impact future research and funding. Available directly to the Penn community are:

1) Optical tweezers instruments for the measurement of nanometer-scale displacements and picoNewton-scale forces, both used for measuring biological forces and manipulating objects in vitro and in the cytoplasm of live cells.

2) A Bruker Catalyst Atomic-Force-Microscope (AFM) for measuring nanoNewton-scale forces, and for imaging microfabricated surfaces.

The laboratories of PMI members also develop and utilize advanced technologies for measuring macromolecule dynamics and localization that may be accessed via collaboration. Unique multiwavelength total internal reflection fluorescence (TIRF) microscopes provide millisecond-scale temporal and nanometer-scale spatial resolution of fluorescent molecules (e.g., GFP-proteins & quantum dots) in vitro and in vivo. In addition, polarization optics allow conformational changes to be observed in single molecules.



Atomic Force Microscopy Core (Penn)



Automated Claims and Medical Record Databases (Penn)

Summary:

ACARD (Automated Claims and Medical Record Databases) is a core in the Center for Clinical Epidemiology and Biostatistics (CCEB) created to foster epidemiologic research into therapeutics, using large population-based automated claims databases (Medicaid / Medicare) and medical records databases (GPRD™ and THIN), and an adverse event reporting system (AERS) database.

Goals:
• Encourage investigators with diverse training to conduct studies focused on translational therapeutics and drug safety
• Provide access to large databases that allow for rapid and cost-effective research on therapeutics and drug safety
• Foster collaboration with CTSA sites outside of Penn who wish to use these databases for clinical research



Behavioral and Physiological Phenotyping Core (Monell)

Summary:

Monell's Behavioral and Physiological Phenotyping Core provides training and research support in the behavioral and physiological phenotyping of rodents, including surgical and electrophysiological techniques utilized in rodent models. Core personnel offer expertise, instruction and equipment needed for methodologies common to research in the chemical senses, including preference tests, gustometers, olfactometers, LabMaster systems and metabolic cages.



Bioinformatics Core (Penn)

Summary:

The Bioinformatics Core of the Institute for Biomedical Informatics (IBI) provides professional bioinformatics services, including data analysis and consultant to UPenn biomedical research community. The core is also dedicated to build efficient pipelines to handle various next-generation sequencing (NGS) data, generated within our NGS core or elsewhere.

The Bioinformatics Core is also supported by IBI members whose areas of expertise include NGS related algorithm development, human disease research (diabetes, cancer, Autism, Alzheimer’s disease etc.), functional genomics, medical informatics, statistics genetics, computational biology. We provide bioinformatics support for grant application in all biomedical areas by drafting approaches, and offering computation resources and expertise for the proposed research.



Bioinformatics Facility (Wistar)

Summary:

The mission of the Bioinformatics Facility is to support the continuing research and education mission of The Wistar Institute and to grow and evolve in response to emerging research needs.

The Bioinformatics Facility is located in the Center for Systems and Computational Biology, which provides a state-of-the-art server room, office space, and educational and conference room space. The Facility provides Cancer Center investigators with database management, software application support, expertise in statistical analyses and computational modeling of biomedical research data and has recently grown to include statistical specialists and programmers as well as computational biologists. The Facility is supported and advised by members of the Center for Systems and Computational Biology.

Functions of the Facility reflect the research requirements of the three Cancer Center programs and are broadly divided into three areas: (i) data-management; (ii) statistical analyses and computational modeling; and (iii) advanced bioinformatics tools for integrative cancer biology. Typical data analyses include large scale information datasets (omics data), generated by high-throughput technologies addressing the following complex area:

• Genome sequencing (alternate splicing, RNA editing, mutation detection)
• Gene regulation (ChIP-chip, ChIP-seq, epigenetic profiling, promoter methylation arrays)
• Biomarkers (e.g. mRNA and miRNA microarray expression data)
• Proteomic analyses (mass spectrometry-based spectra, LCMS, DIGE, etc.)
• Polymorphism genotyping (e.g. Single Nucleotide SNP and Copy Number variations CGH, LOH).

The Facility has placed a high priority on integrating cancer research information representing a variety of data types, including clinical data, microarray data, massively-parallel sequence data, protein data, RT-PCR and functional assays. Data security is a primary focus of the Bioinformatics Facility in designing and implementing software systems.



Biological Chemistry Resource Center (Penn)

Summary:

The Biological Chemistry Resource Center (BCRC) at the Department of Chemistry has been established to provide an open access user facility for state-of-art biophysical analytical instrumentation. The goal of the center is not only to provide access to instrumentation, but also supply the graduate student and post-doc user community with a firm understanding of the scientific principles behind the techniques and on-site expertise to ensure successful experimentation. Instrumentation access will be available to the entire University of Pennsylvania research community.



Biomechanics Core Facility (Penn)

Summary:

The Biomechanics Core works with ITMAT faculty from Penn, ITMAT partner institutions, and members of the ITMAT Program in Translational Biomechanics."

"Consultation and initial pilot experiments performed with the Biomechanics Core are free-of-charge to ITMAT faculty from Penn, ITMAT partner institutions, and members of the ITMAT Program in Translational Biomechanics.



Biorepository Core (CHOP)

Summary:

The Biorepository Core collects and organizes biospecimens from investigators across the Research Institute. With a capacity for approximately 7 million samples, the facility is designed to house all of the biospecimens available at Children's Hospital, avoiding specimen duplication, preserving precious materials, and providing broad access to data and materials. Initial sample collection will focus on DNA samples, but with the addition of other freezers in the near future, the facility can also safely store fluids, RNA, tissue samples, and a number of other biospecimens.



Biostatistics Analysis Center (Penn)

Summary:

The Biostatistics Analysis Center (BAC) provides biostatistical and epidemiological consulting services to the University of Pennsylvania Health System research community. The BAC is staffed by professionally trained biostatisticians, biostatistical programmers, Geographical Information Systems (GIS) experts, data managers, and data entry staff. Faculty oversight for the BAC is provided by members of the CCEB Biostatistics and Epidemiology Divisions, with leadership for daily operations provided by a dedicated staff-level director. Specifically, Warren B. Bilker, PhD, Professor of Biostatistics, and John T. Farrar, MD, PhD, Associate Professor of Epidemiology, are Faculty Co-Directors and Amy Praestgaard, MS, is the staff-level BAC Director.

• Request BAC statistical analysis, programming, GIS, or data management services by completing the CCEB Research Services Request Form.
• Request a BAC Statement of Work and budget for inclusion in a grant submission by completing the CCEB Grant Registration Form and indicate in the Collaborative Personnel and Resource Needs section that you require MS Biostatistician support.



Biostatistics and Data Management Core (CHOP)

Summary:

The Biostatistics and Data Management Core (BDMC) at The Children's Hospital of Philadelphia (CHOP) supports investigators from virtually all subspecialties of pediatric medicine and supports studies ranging from small, narrowly defined basic science projects to large, multi-site clinical trials.

The Biostatistics and Data Management Core currently supports more than 50 funded studies and collaborates with investigators on numerous grant applications each year. The BDMC is staffed by a Scientific Director, Deputy Director, and data management/information technology managers, as well as approximately 20 additional staff members representing the disciplines of biostatistics, data management, information technology and administration. The BDMC is located on the CHOP campus (3535 Market Street), and is operated and supported by Westat, a large health research organization with extensive biostatistics, data management and information technology capabilities.



CHOP Microbiome Center (Sequencing and Analytical Resource) (CHOP)

Summary:

The CHOP Microbiome Center supports planning microbiome projects, DNA purification, library preparation, high throughput sequence analysis, and bioinformatic analysis of the output."

"To request pricing or core services, please complete this form and send to:

Jessi Erlichman erlichman@email.chop.edu

Requests for pricing must be submitted at least 4 weeks prior to grant deadline.



CTRC Behavioral Neurosciences Core (CHOP)

Summary:

The Behavioral Neurosciences Core provides consultation and assistance to investigators regarding psychological, neuropsychological, and psychiatric components of research studies involving pediatric subjects.

Together, the services provided by the Behavioral Neurosciences Core offer research infrastructure support across the entire process of research, from pre-design consultation to development and analysis of behavioral data, and across multiple domains of functioning and outcomes.



CTRC Cardiovascular Phenotyping Unit (Penn/CHOP)

Summary:

The Cardiovascular Phenotyping Unit provides cardiac testing services with shared facilities for both children and adults. The Unit provides the technical services and expertise to conduct the highest quality research, provides research tests in a cost-effective manner, and provides unparalleled training opportunities in clinical research for investigators, fellows, students and technicians. Most services are provided across the life cycle. For studies involving both adult and pediatric populations, the pediatric and adult CPUs can collaborate closely and standardize procedures according to the investigators’ needs. Please contact the Directors for special arrangements or input regarding cardiovascular phenotyping.



CTRC Exercise Medicine Unit (Penn)

Summary:

The Exercise Medicine Unit offers exercise training and testing services. The exercise training room contains:

• treadmills,
• arc trainer,
• recumbent bike,
• functional trainer,
• adjustable bench,
• and a power block area.

It is staffed by a full-time, certified exercise trainer who can help design exercise training protocols and administer them to clinical research study participants. Exercise interventions can be designed to occur onsite at the Mutch building or for community/home settings. Consultations to assist with design of exercise intervention protocols are available.

Exercise testing services include a recumbent exercise bike and a motorized treadmill, with adjacent ECG monitoring, metabolic cart and VO2 max measurements, and anaerobic power testing. Additional testing services include grip strength, six minute walk tests, and gait speed. Objective Physical Function tests (e.g. SPPB, TUG, and PPT) can also be done by the staff of this unit. Consultations to assist with designing exercise testing protocols are available.



CTRC Informatics Services Core (Penn/CHOP)

Summary:

The staff of this core gives guidance and/or coordinates data management, capture and analysis on behalf of the investigator within the Institute and assists more broadly with computing issues related to the approved protocol. This core provides services to investigators for their CTRC-approved studies that are utilizing other CTRC core services.



CTRC Nutrition Core, Dietary Assessment (Penn/CHOP)

Summary:

Nutrition plays a vital role in health at all ages. The Clinical and Translational Research Center offers a Bionutrition Research Unit (BRU) to facilitate and implement clinical and translational research services. Research dietitians assist investigators with research design, implementation, data collection and analysis in study protocols.

The Dietary Assessment Unit of the Nutrition Core provides a broad range of nutrition-related research services to investigators at the Children's Hospital of Philadelphia, the Hospital of the University of Pennsylvania (HUP) and Penn Presbyterian Medical Center (PMC).



CTRC Nutrition Core, Nutrition Assessment (CHOP)

Summary:

The Nutrition Assessment Unit of the Nutrition Core is a state-of-the-art facility for the assessment of growth and body dimensions, body composition (the amount of muscle, fat and bone in the body), energy expenditure, bone density, and muscle strength. The Unit has two locations and four experienced technicians for performing research assessments.



CTRC Ophthalmology Core (CHOP)

Summary:

The Clinical and Translational Research Center (CTRC) offers ophthalmological testing services for children. The Ophthalmology Core at CHOP’s CTRC was established to provide clinical and translational research services in ophthalmology for the assessment of visual function and structure.

Services provided include:
• Eye exams (includes visual acuity, recognition acuity, grating acuity, motility, slit lamp exam / anterior segment evaluation,
external segment evaluation, fundus exam, refraction, best corrected)
o Contrast sensitivity
o Color vision testing
• Optical coherence tomography (OCT) tests of the:
o Anterior segment
o Posterior segment – optic nerve
o Posterior segment – retina
• Visual field measures:
o Using Humphrey
o Using Goldman
• Full field sensitivity testing
• Electroretinography
• Visual evoked potential
• Fundus photography
• Ocular ultrasound
• Professional interpretation of all tests is also available



CTRC Research Nurse Core (Penn/CHOP)

Summary:

The Clinical and Translational Research Center (CTRC) has two main locations as well as satellite locations. The protocols cover a wide variety of research areas including: HIV, sleep disorders, cholesterol, obesity, diabetes, various cancers, arthritis, hypertension, renal disease, short bowel syndrome, and neonatal and surgical studies as well as new treatments for various diseases. The CTRCs service over 1200 inpatients and over 6000 outpatients a year. Research subjects range from premature infants to the elderly, with the majority of adults being seen at HUP.

HUP Unit - Dulles Building:
• 8 bed inpatient
• 8 chair and 2 bed outpatient unit
• metabolic kitchen
• Scatterbed nursing services throughout hospital units including the ICUs, ED and operating rooms

UPPMC Unit – 1st Fl Mutch Building:
• 18 outpatient treatment beds
• metabolic kitchen

CHOP Units:
• 4 bed inpatient unit – 5 West Main
• outpatient unit with 2 treatment rooms, 4 treatment chairs and a consultation room - Main 7
• Scatterbed nursing services in Newborn Nursery - Ravdin Building

The nurse manager should be contacted prior to submitting a new protocol submission to the CTRC and discussions should continue throughout the start-up process.



CTRC Sleep Core (PENN/CHOP)

Summary:

The CTRC Sleep Core provides services in support of clinical sleep research. It is based at two sites: the CHOP Sleep Laboratory, convenient to the CTRC Outpatient Facility on the 7th Floor of Main Hospital and the University of Pennsylvania’s Sleep Laboratory, located on the 11th Floor of the Gates Building which is part of the medical complex of the Hospital of the University of Pennsylvania. The Sleep Core contains a total of six designated research beds, dedicated staff, and state-of-the-art equipment that provides support for a variety of sleep-related research initiatives. Studies performed in the Sleep Core include overnight polysomnography, multiple sleep latency testing, neurobehavioral testing and actigraphy. The Sleep Core´s goals include providing highest-quality sleep studies, extending sleep research to disciplines not traditionally involved in this area, further developing extant multidisciplinary programs, and offering training opportunities for medical students, residents, fellows, and junior faculty in clinical sleep research. The Sleep Core is associated with CHOP’s and UPHS’ American Academy of Sleep Medicine-accredited Sleep Center Laboratories.

Services for pediatric and adult subjects:

Study Design
Overnight Polysomnography
-Polysomnography Interpretation
Multiple Sleep Latency Testing
Actigraphy
Neurobehavioral testing
Sleep Core Library



CTRC Study Design and Biostatistics Core (Penn/CHOP)

Summary:

The Study Design and Biostatistics (SDAB) Core works closely with existing resources to provide targeted study design and biostatistics support to ITMAT/CTSA investigators. The Core serves as a direct provider of services, including protocol review, study design, proposal development, and performance of simple to potentially substantial complex analyses. SDAB integrates the support available with the HUP and CHOP Clinical and Translational Research Centers (CTRCs), the expertise and resources of faculty in the Center for Clinical Epidemiology and Biostatistics / Department of Biostatistics and Epidemiology (CCEB/DBE), the Biostatistics Analysis Center (BAC), and the Biostatistics and Data Management Core (BDMC) at CHOP.



CTRC Translational Core Laboratories (Penn/CHOP)

Summary:

The Translational Core Laboratory consists of the Specimen Collection, Processing and Point of Care, Biochemistry, Cell Culture/DNA Isolation, and Molecular Biology core laboratories. Laboratory testing is integrated across Penn and CHOP, and TCL services are provided at multiple physical locations at both Penn and CHOP.

Penn location: first floor Smilow Center for Translational Research
CHOP location: 804 Abramson Research Center (ARC)



Cancer Histology Core (Penn)

Summary:

The Cancer Histology Core is a non-profit, research-oriented resource core supported by the Abramson Cancer Center. It offers all histology-related services to all members of the Abramson Cancer Center with high quality, low cost, fast turnout, and easy interaction.

Service will be open to all life sciences investigators at Penn, but priority will be given to full members of the Abramson Cancer Center.



Cartographic Modeling Lab (Penn)

Summary:

The CML specializes in applying geographic information systems (GIS) software and hardware to digitally link data and geography to generate spatial databases, maps, spatial statistical analyses, and mapping applications, providing a useful way to reveal spatial and temporal relationships among data.

By using GIS to visualize geographic relationships that affect health outcomes, public health risks, disease transmission, access to health care, and other public health concerns, the CML conducts spatial research, policy analysis, and develops mapping applications of value to investigators at Penn and beyond.



Cell Center Services Facility (Penn)

Summary:

Cell Center Services Facility is the service component of the Cell Center, provides training and services in various cell culture and associated procedures including Mycoplasma and Endotoxin testing. The cell culture service includes cell culture at various scales, large scale growth of hybridoma and other cell lines followed by antibody purification by protein G column and the generation of lymphoblastoid cell lines by EBV induced transformation of lymphocytes. In addition, the facility prepares specialized cell culture media, drosophila media, and various molecular biological reagents.

Recently cell transfection and selection service has been introduced at the facility.



Cell Center Stockroom (Penn)

Summary:

The Cell Center Stockroom is a division of the Genetics Core Facilities (GCF). The GCF is a University service center, established in 1973 to provide consultation, training, and services in the areas of cell culture and hybridomas. Also, the GCF to provides a full range of cell culture media and molecular biology reagents needed by investigators to perform cell culture techniques in their own laboratories. The DNA Sequencing Facility, Genetic Diagnostic Laboratory and Transgenic/Chimeric Animal Facility are the remaining three divisions of the GCF.

The Stockroom serves University of Pennsylvania investigators and affiliate institutions (Cancer Center, Chidren's Hospital of Philadelphia, Hospital of the Unviersity of Pennsylvania, The Wistar Institute, Monell Chemical Senses Center, and Presbyterian Hospital) by coordinating relations with various suppliers of molecular biological research materials. This involves not only bulk purchasing of these products, but the negotiation of discounts and convenient delivery arrangements. There are over 1,100 products on-site for immediate delivery in the Stockroom. Special ordering of non-regularly stocked products is available from 28 bioreagent vendors with discounted pricing and overnight delivery.

List of Stockroom Vendors:

Amaxa Biosystems
Ambion, Inc.
Applied Biosystems
Atlanta Biologicals
Bio-Rad Laboratories
Biowhittaker
Cell Center Services
Cell Signaling Technologies
Clontech Laboratories
Collaborative
Denville Scientific
Difco Laboratories
EMD Millipore
Fermentas
Fisher (Thermo) Scientific
GE/Amersham Bioscience
Gemini Bio-Products
Hyclone Laboratories
Integra Biosciences
Integrated DNA Technologies Invitrogen
ISC Bioexpress
KAPA Biosystems
Kodak
Life Technologies
Lonza
MediaTech
New England Biolabs
Open Biosystems
Perkin Elmer Life Sciences
Promega
Qiagen
Rainin Instruments
Roche
Sigma-Aldrich
Stratagene/Agilent Technologies
USA Scientific
USB Corporation/Affymetrix
VWR International
WorldWide Medical Products



Cell and Developmental Biology Microscopy Core (Penn)

Summary:

The CDB Microscopy Core is the primary light microscopy facility for researchers at the Perelman School of Medicine at The University of Pennsylvania. We are also open to the entire University of Pennsylvania community as well as to CHOP, Wistar, and other local institutions. While our emphasis is on confocal and related technologies, our aim is to provide personalized assistance on all aspects of imaging, from tips on sample preparation to training on one of our microscopes to processing and analysis of image data.



Center for Advanced Computed Tomography Imaging Services (Penn)

Summary:

Radiologists, physicists, and technologists help researchers utilize the resources available within the Department of Radiology at the University of Pennsylvania. Our mission is to oversee proposed research protocols that involve human, animal, phantom or specimen studies in an effort to achieve two goals:
• To ensure that all research performed on the CT scanners comply with CACTIS and University policy, and Federal Regulations
• To determine if CACTIS can maintain the resources required to carry out each research protocol, including personnel, software, hardware and scan time

Under the direction of the Chair, Dr. Harold Litt, the CACTIS committee reviews proposed research requests and makes decisions and recommendations accordingly.

Additionally, CACTIS:
• Oversees the day-to-day operations of all CT procedures associated with research protocols
• Provides information regarding the use of the CT facilities to the research community at the University of Pennsylvania
• Provides CACTIS users with all of the policies of the institution governing research
• Ensures that CACTIS is in compliance with these policies



Center for Advanced Magnetic Resonance Imaging and Spectroscopy (Penn)

Summary:

Mission
The overall mission of CAMRIS is to provide oversight in the responsible use and application of Magnetic Resonance in research through leadership, education, and guidance. These principles are manifest in the development of new research and collaborations inside and outside the Radiology Department which can translate into advanced clinical techniques; training in safe and efficient use of this investigative tool and dissemination of current, accurate and evolving MR Technology; scheduling upgrades of MR Systems and facilities; scheduling systems operations and personnel within the MR department; and receiving and acting on recommendations pertaining to the administration of CAMRIS Facilities.



Center for Applied Genomics (CHOP)

Summary:

CAG offers next-generation sequencing (NGS), SNP genome wide association study (GWAS), and a range of other services.



Center for Injury Research & Prevention Core (CHOP)

Summary:

The Center for Injury Research and Prevention is dedicated to advancing the safety and health of children, adolescents, and young adults through comprehensive research resulting in practical tools to reduce injury and promote recovery.

To advance science and create tangible impact, the Center:

Addresses children's injuries comprehensively - from before-the-injury prevention to after-the-injury healing
Translates rigorous scientific research to usable, age-appropriate tools and practical steps for families, professionals, and policymakers
Asks and answers important questions from an interdisciplinary perspective, with expertise in Behavioral Sciences, Clinical * Care, Engineering, Epidemiology and Biostatistics, Human Factors, Public Health and Communications
Engages with a broad range of organizations from universities and government entities to non-profit groups, foundations and corporations, to ensure that research results extend to the real world
CIRP turns "research into action" by determining priorities for pediatric injury research, establishing key collaborations and networks to apply that research, and providing education, training and professional development across three injury science disciplines: Behavioral Science, Engineering, and Epidemiology and Biostatistics. The Center also utilizes Outreach and Dissemination to translate the research across these disciplines into real-world applications.



Chemosensory Receptor Signaling Core (Monell)

Summary:

This Research Core will provide training, advice and research support in molecular biological techniques used to analyze gene expression in cell culture and manipulate the genomes of rodent model organisms. The Core will centralize labor-intensive construct generation common to multiple users and provide users with technical expertise in molecular biological manipulations both in vitro and in vivo. The Core facility has all equipment, reagents, and expertise needed to carry out the following manipulations of RNA, DNA, and protein: RNA isolation, cDNA production, antisense RNA amplification, cloning, subcloning, recombineering, gel electrophoresis, transfection and tissue culture.



Clinical Cell and Vaccine Production Facility (Penn)

Summary:

The Clinical Cell and Vaccine Production Facility (CVPF) renders bench-to-bedside translational medicine a reality. Equipped with state of the art facilities, the CVPF manufactures cell and gene biotherapeutics and is accredited by the Foundation for the Accreditation of Cellular Therapy (FACT). Further, the CVPF is the only GMP (good manufacturing practices) compliant facility on campus and functions as an NCI approved Abramson Cancer Center (ACC) Shared Resource. As an ACC Shared Resource and Path and BioResources core facility, the CVPF supports numerous investigational new drug (IND) protocols. Current protocols target a variety of disease indications (primarily HIV, adult and pediatric cancers, and stroke); many more trials are in development and, once approved, will further expand the scope of diseases targeted for cell and gene therapy. For more information on current trials, explore our “Clinical Trials” page.



Clinical Research Computing Unit (Penn)

Summary:

The Clinical Research Computing Unit (CRCU) is an Academic Clinical Research Organization within the Center for Clinical Epidemiology and Biostatistics (CCEB) in the Perelman School of Medicine at the University of Pennsylvania. Since its inception in 1997, the CRCU has been expertly providing the full range of services essential for the conduct of clinical research projects, including Phase I-IV, multi-center, randomized, clinical trials, registry, and cohort studies utilizing state-of-the-art technology and tools to ensure superior data quality. The CRCU provides expertise in project management, data coordination and research computing tailored to meet your project requirements. The CRCU project teams partner with the BAC in the project design phase to plan accurate and precise data collection modules and to structure project reports for steady oversight. We specialize in study design and development, site management and training, data collection, processing, quality control, regulatory requirements and reporting, database development, administration, security, data storage and proposal development.



Clinical Research Support Office (CHOP)

Summary:

The Clinical Research Support Office (CRSO) is the central office for clinical research support services at CHOP. The CRSO provides leadership, administrative guidance, and support services to support both novice and experienced clinical investigators with investigator-initiated and industry-sponsored research projects. The CRSO’s core services include clinical research professionals who support operationalizing clinical research projects. These services include RNs, CRCs, and PMs to operationalize and manage clinical research projects; regulatory affairs professionals to prepare, submit, and manage INDs, IDEs, and other submissions to regulatory agencies and committees; contracting and legal professionals to negotiate agreements in support of clinical research; recruitment and marketing professionals to work with investigators to strategize, develop, and implement marketing and recruitment plans to facilitate enrollment into clinical research projects; and other business and administrative support.

The mission of the CRSO is to make it easier for the CHOP research community to conduct quality clinical research projects. The CRSO’s services include:

• Clinical research personnel (study coordinators, research nurses, program/project managers, clinical research supervisors) provide support to clinical investigators, enabling investigators to carry out all types of clinical research projects in a manner consistent with CHOP’s mission—excellent patient care, top-quality education, and innovative research. The CRSO assists with the start-up, execution, and completion of clinical research projects and ensures compliance with local and federal requirements. CRSO personnel are well-trained clinical research professionals who can be assigned to support any type of clinical research project.
• Regulatory affairs professionals provide regulatory guidance, operational support, and institutional oversight for clinical trials conducted under a CHOP sponsor-investigator IND/IDE. The CRSO also coordinates and submits regulatory correspondence to regulatory committees and agencies such as the IRB, FDA, and DSMBs.
• Contract administrators negotiate Clinical Trial Agreements (CTA) for industry-sponsored clinical research.
• Recruitment Enhancement Core (REC) collaborates with research teams to strategize, develop, and implement marketing and recruitment plans to increase enrollment into clinical research projects. The REC liaises with other clinical and research departments to leverage institutional biobanking and data repository resources with the goal of building a robust and efficient sample and data biorepository.
• Research navigation services guide and connect research personnel to answers, resources, and tools to facilitate clinical research. The navigator is available to assist investigators with any questions that arise during the design, start up, and execution of clinical research projects.
• Clinical research professionals will assist the research community with the development of clinical research budgets, operational review of investigator-initiated protocols, and other administrative-related activities.

Contact hodgsonm@email.chop.edu with details for a fee quote.



Community Engagement and Research Core (Penn)

Summary:

Mission:
The purpose of the Community Engagement and Research Core in the Penn CTSA is to facilitate community-based research and community engagement, especially community-based participatory research, and enhance the translation of research and technological developments to key public health and community stakeholders.

Goals:
1. Foster community-based participatory research projects through developing training programs and integrating lectures into existing academic programs
2. Determine community health needs and priorities
3. Promote community-based research within the area of health disparities through seminar series
4. Continue the involvement in community outreach and education events to engage the community
5. Fund the conduct of CEAR Core pilot studies
6. Facilitate the use of academic-community partnerships to aid in the recruitment of subjects



Community Outreach Using Health System Informatics Core (Penn)

Summary:

Mission:
To promote the use of Penn Health System information resources in support of clinical research.

Goals:
1. Facilitate collection of data from operational information systems in the Penn health system
2. Facilitate the creation of interventions in operational information systems in the Penn health system
3. Foster the use of information systems such as electronic medical record and computerized order entry in the conduct of clinical trials
4. Enable the use of Electronic Health Records, Computerized Order Entry Systems, Health System Administrative Databases, laboratory and other ancillary test information systems to provide primary data for epidemiological and health services research studies
5. Educate ITMAT investigators on the types and quality of data and limitations of its use for health system information systems



Cyclotron Facility (Penn)

Summary:

One of the cyclotrons is a Japan Steel Works (JSW) BC3015 30 MeV machine, capable of accelerating protons, deuterons, 3He, and 4He. Beam currents of 10-20 mA are typical with a maximum current capability of 60 mA. In 2009, an IBA 18/9 MeV Cyclone cyclotron was added into an expanded vault adjacent to the existing JSW cyclotron. The second cyclotron provides for higher beam currents than are available on the JSW cyclotron. As a result, the 18FF- production yield increases from 2 Ci to 12 Ci and; 11CCO2 yield increases from 3 Ci to 4 Ci, thereby increasing yields of research radiotracers. While the JSW can only irradiate one target at a time, the IBA is capable of irradiating two targets simultaneously and has been the main workhorse cyclotron. In addition to 18FF- production, this cyclotron is also used for 18FF2 bombardment for electrophilic radiosyntheses. Despite its older design and lower yields, the JSW has an advantage of a higher particle energy and capability to produce alpha particles; this is a rare and valuable asset and enables our facility to produce novel radionuclides for biomedical research. In particular, the JSW currently is used to produce At-211, a radionuclide that has potential in targeted systemic radiotherapy.

The facility is divided into two sections: a clinical production laboratory where the radiopharmaceuticals used in routine diagnostic scans and clinical studies are produced, and a multiuse research area in which new radiotracers are developed for cell studies, animal studies and other research uses."

"The clinical production laboratory is operated under cGMP regulations.



DNA Sequencing Facility (Penn)

Summary:

The DNA Sequencing Facility provides reliable, long read, automated Sanger sequencing with fast turnaround; microsatellite-based genotyping and fragment analysis; plasmid and BAC DNA preparation and purification; and related molecular biological services including PCR, cloning, sub-cloning, site-directed mutagenesis, and preparation of targeting vectors for gene targeting in mice. It also provides services and support for analysis and interpretation of sequence data as well as the design of approaches to complex sequencing projects.

For the last four years the facility has been providing Roche 454 sequencing service that includes library preparation, emulsion PCR and pyrosequencing for both genomic DNA and amplicons. Data analysis is provided in each project depending on the investigator’s specific need.

Ion Torrent's Personal genome machine (PGM) is the latest addition at the facility. Known for scalability, simplicity and speed, this inexpensive technology is advancing fast to achieve new goals in terms of throughput and read length. The maximum read length and the throughput available at this point is 200 b and 1 Gb respectively. The applications are similar to those of long-read 454 sequencer and includes targeted resequencing of barcoded samples, sequencing of captured library, sequencing of bacterial and viral genomes, sequencing of metagenomic samples, RNA-seq specially small RNA sequencing and validation of sequence data obtained on other platforms. The sequencer comes with Torrent Suite, the Torrent server analysis pipeline that is the primary software used to process raw data acquired by PGM sequencer to produce sequence read files. The base calls are in both SFF and FASTQ file formats for easy downstream analysis with third party analysis tools. The Torrent suite performs filtering, trimming, mapping with the generation of a Variant Caller report. This long read sequencer is going to bring down the cost of new generation sequencing significantly.

The range of services mentioned above along with the expertise of the facility personnel enables this core to provide full support for investigators at Penn, who can easily obtain fast, reliable data on genes of interest, whether they are doing targeted or whole genome tumor genome sequencing, deep resequencing, screening clones for sequences of interest, establishing the identity of new clones, or searching for mutations in specific genes.



Electron Microscopy Resource Laboratory (Penn)

Summary:

The EMRL is a full service, shared resource facility at the University of Pennsylvania’s School of Medicine. The EMRL primarily serves the biomedical research community at the University of Pennsylvania but offers high quality EM imaging services to researchers beyond Philadelphia and academia. Our staff of experienced professionals performs routine TEM, SEM, single particle and tomographic image reconstructions, and image analysis of all types of biological material. The facility is well equipped with 3 TEMS: a JEOL1010 with a 1K x 1K video rate AMT digital camera, a FEI Tecnai12 120KeV S/TEM microscope which is equipped with a 2K x 2K Gatan 894 camera and EDAX electron dispersive SiLi detector, and a FEI TF20 200 KeV S/TEM microscope with the latest generation CMOS direct electron detector, 2K x 2K video rate Gatan Orius camera as well as a Fischione HAADF for STEM Z imaging. The facility also houses a FEI Quata250 environmental SEM.



Flow Cytometry Core Laboratory (CHOP)

Summary:

The Flow Cytometry Core Laboratory provides access to state of the art instrumentation and professional flow cytometry services to members of the research community of The Children's Hospital of Philadelphia and University of Pennsylvania; investigators from outside the campus are welcome to our facility. The lab has space on the 12th floor of the Leonard and Madlyn Abramson Pediatric Research Center and on the fourth floor in Colket Translational Research Center. The staff has the required expertise for performing a variety of flow cytometry applications, including but not limited to sample processing for surface and intracellular staining, functional assays, complex multi-color flow cytometry analyses and cell sorting.



Flow Cytometry Facility (Wistar)

Summary:

The Wistar Institute Flow Cytometry Shared Resource provides flow cytometric services, training, advice, and support for the use of flow cytometric techniques by Wistar Cancer Center investigators, as well as the greater basic research community.



Flow Cytometry and Cell Sorting Resource Laboratory (Penn)

Summary:

The Flow Cytometry and Cell Sorting Resource Laboratory is currently recognized as one of the largest and most comprehensive flow cytometry laboratories in the US. In 2010 it was designated a laboratory of exceptional merit by the National Cancer Institute. Using state-of-the-art technology, the resource provides a broad array of, instrumentation, support, education and consultation to the research community at the University of Pennsylvania. A wide variety of cell sorting applications are supported, from high-speed multicolor (up to 14 colors) cell sorting to low-speed, large nozzle, improved viability sorting. Additionally, a wide variety of cell analysis services (up to 20 parameters) are offered, from traditional analog, easier to use tabletop analyzers to many-laser, many-color, high-speed, fully-digital modern instrumentation. Currently the facility offers 6 cell sorters and 19 analytical instruments. A very active training and consultation program is in place to support these activities. The Scientific Director, Dr. Jonni Moore, and the Technical Director, each have over 25 years experience in the field of cytomics. Researchers at the University of Pennsylvania are increasingly engaged in research projects that require 8-plus-parameter cell sorting of infectious cells and primary human tissues. Investigators using the Flow Cytometry and Cell Sorting Shared Resource have access to virtually any type of cytometric services required for a vast array of applications.



Genetic Diagnostic Laboratory (Penn)

Summary:

The Genetic Diagnostic Laboratory is a non–profit laboratory at the University of Pennsylvania. Established in 1994, the Genetic Diagnostic Laboratory has had the pleasure to serve patients, physicians, and other members of the medical and research community in many states in the U.S., as well as in over 24 countries worldwide.

Our mission is to evaluate an individual's DNA to discover a genetic cause for their disease or physical symptoms, provide interpretation of the genetic finding and its association with disease, develop new methods for analyzing genes, and introduce new testing to improve patient care.

The Genetic Diagnostic Laboratory is CLIA certified and has state permits for California and Maryland. The staff of the Genetic Diagnostic Laboratory includes highly trained and experienced laboratory technicians, as well as a genetic counselor, who work continually to provide their services in a timely and professional manner.



Genomics Facility (Wistar)

Summary:

The Wistar Genomics Facility serves as a hub for consultation and scientific interactions relating to nucleic-acid based methods and provides expertise and support to insure the best possible use of emerging nucleic-acid technologies.

In addition to consultation and collaboration with Wistar Cancer Center members, the Facility provides services to the greater scientific community.

The establishment of this facility was supported in part by an NCI Cancer Center Support Grant and equipment grants from the Commonwealth of Pennsylvania, The Pew Charitable Trusts and the National Cancer Institute.



Healthcare Analytics Unit Core (CHOP)

Summary:

The Healthcare Analytics Unit (HAU), a core facility of CHOP’s Research Institute that is co-directed by CPCE and PolicyLab, administers these resources. HAU’s staff has expertise in managing and using various data sources, ranging from electronic health records and clinical trial or registry data to administrative, claims, or survey data.

The HAU serves as a resource for CPCE, PolicyLab, and other CHOP investigators using complex data to address research questions. HAU provides services in data extraction and management, statistical programming, biostatistics analysis, and analytics data consultation.



High Throughput Sequencing Core (CHOP)

Summary:

The Children's Hospital of Philadelphia and the Beijing Genomics Institute (BGI) have formed a collaborative genome center entitled BGI@CHOP. Together, these elements form The High-throughput Sequencing (HTS) Core with increased capacity, expertise and analytical resources for conducting next-generation sequencing studies. The HTS Core will provide automated library construction and high-quality, high-throughput sequencing services for whole genome and whole exome samples.



High-Throughput Screening Core (Penn)

Summary:

The High-throughput Screening (HTS) core provides the Perelman School of Medicine community with professional HTS screening services to identify genes or organic small molecule modulators of signaling pathways, cellular phenotypes, and protein function in models of human disease. Core staff will educate and assist scientists with HTS assay development, optimization, miniaturization, and validation; maintain libraries of siRNA, shRNA, cDNA, and FDA approved/FDA-like organic small molecule libraries for HTS; and provide robotics infrastructure and technically trained staff for HTS, including small screens of user defined libraries. The High-throughput Screening core also provides direct assistance with preparation of grant applications by drafting experimental designs approaches and providing Letters of Support, offering HTS resources and analysis expertise for the proposed research.



High-performance Computing (Penn)

Summary:

The PennHPC facility opened in April of 2013 to meet the increasing growth in genomics processing and storage, as well as growth in other scientific areas requiring computational capacity such as imaging and biostatistics/bioinformatics. The cluster is managed by two fulltime system administrators, and is located at the Philadelphia Technology Park, a Tier-3, SSAE 16/SAS 70 Type II Audit compliant colocation/datacenter facility.



Histology and Cellular Localization Core (Monell)

Summary:

The Histology and Cellular Localization Core provides training and research support in microscopy, anatomy and histology of chemosensory systems. Core personnel work with Monell researchers to develop and optimize in-house procedures and to establish cutting-edge techniques in histology and cell anatomy. The Core provides centralized services such as tissue sectioning and in situ probe preparation. The Core facility is equipped with cryostats, fluorescence microscopes, confocal microscopes, and a two-photon microscope.



Histology and Gene Expression Core (Penn)

Summary:

The Histology and Gene Expression Core Facility provides expert professional services for members of the Cardiovascular Institute. Services for non- Institute investigators are available on a fee-for-service basis as time permits. The Histology and Gene Expression Core offers all histology-related services include tissue processing, embedding, sectioning, staining, immunocytochemistry and InSitu Hybridization.



Histotechnology Facility (Wistar)

Summary:

This facility provides basic histology services. These include fixing, processing and paraffin embedding of all types of tissues for light microscopy (i.e. routine stains, immunohistochemistry or in situ hybridization). Routine hematoxylin and eosin staining as well as special staining is done in the lab. Slides are prepared for immunohistochemistry and in situ and immunohistochemistry.

Frozen sectioning is also available. It is advisable to contact the facility about freezing techniques so the best sections can be obtained.



Human Immunology Core (Penn)

Summary:

The Human Immunology Core provides reagents and scientific expertise to investigators studying immune function in humans. The core serves as a central facility for cell and tissue processing, generation of human blood cell products, and the performance of qualified cellular and molecular immune assays for early-phase clinical trials. Assays include multicolor immunophenotyping, high throughput sequencing of T cell receptor and B cell receptor genes, luminex, ELISPOTs, ELISA and expert scientific and technical consultations to investigators wishing to develop or incorporate the newest immunology technologies into their research.



ITMAT Bioinformatics Facility (Penn)

Summary:

The ITMAT Bioinformatics Facility provide project based bioinformatics support for ITMAT translational researchers. Our focus has been on providing the computational infrastructure and programming support needed to conduct high-throughput proteomics experiments. We also support other genomics high-throughput technologies to a lesser extent. See the resources page for more information on our projects and what we have to offer.
The projects range from building easy to use Web applications for data analysis pipelines, one-off scripting, clinical and basic science research support, algorithm development. Recent efforts have focused on explorations of new models of computation, specifically Cloud Computing and GPUs, for use in genomic scale research. Feel free to contact us for more information.



Imaging Facility (Wistar)

Summary:

The Imaging Facility is a shared resource with the primary goal of providing exceptional microscopy and imaging services, as well as individual access to a variety of state-of-the-art imaging resources for members of the Wistar research community. The imaging systems have been designed to be extremely flexible to reflect a broad range of challenging scientific questions and specimens. Each system provides a combination of illumination, optics and image capture options. Diverse subjects ranging from fluorescently tagged live cell cultures and stained tissue sections, to 3D tumor spheroids and low magnification explanted tissues, can be accommodated with available systems.

Current equipment includes standard upright and inverted fluorescence microscopes, a customized live-cell time lapse microscope capable of 6D imaging, a laser scanning confocal microscope, a 2-photon microscope designed for in vivo imaging, a small animal, whole body luminescence and fluorescence imager, special low magnification (photomacrography) systems as well as a variety of traditional photographic cameras, lenses and lighting equipment. Users of the facility may be trained for unassisted use of all core assets, or they may elect assisted service with the facility staff performing the imaging.

The Imaging staff also provides assistance to researchers with additional aspects of their imaging requirements. Ideal approaches to specimen documentation are often unique to the experiment and the staff can help design the most effective imaging protocols to answer a particular question. On-site assistance is available to help investigators get the most out of their own systems. Image analysis and specialized Photoshop training, creative imaging for journal covers, and guidance on digital imaging ethics help to round out the services available from the facility.



Immunology Core (Penn)

Summary:

Immune responses to products of viral vectors have posed formidable barriers to efficient gene therapies. The important immune effectors of the immune response include CD4+ T helper cells, CD8+ cytotoxic T cells, which are responsible for mediating elimination of transgene expression and B cells which produce neutralizing antibodies that block effective readministration of vector. In addition immune responses directed to neoantigens expressed by the transgene in vector-transduced cells, are also responsible for the rapid elimination of transgene expression. The Immunology Group is responsible for performing various assays to evaluate both cell mediated- as well as humoral immune responses in animal models of gene therapies. In this respect, the Group has undertaken analyses of immune responses in pre-clinical trials in gene therapy in mice, rats, rhesus monkeys, and dogs and in several clinical trials. These assays monitor adenovirus-, adeno-associated virus- and transgene-induced cell mediated and humoral immune responses.

The following schematic illustrates the various immunological processes for which the Immunology Group has developed methodologies:
schematic from core website
Figure Legend: Antigen taken by antigen presenting cells (APC) is processed and presented by MHC class I to CD8 T cells , and MHC class II to CD4 T cells . Recognition of the antigen, along with costimulatory molecules (B7-CD28; CD40-CD40 ligand) results in activation of antigen-specific CD4 T cells, which leads to lymphoproliferation and cytokine secretion . Depending on several conditions (e.g. strength of antigen signaling, costimulation, cytokines secreted by APC, etc.) CD4 T cells differentiate into either TH1 or TH2 type cells. TH1 cells secrete predominantly IFNg (interferon-gamma), which plays a role in activation of cell mediated immune responses which culminates in activation of cytotoxic T lymphocytes . CTL have been shown to be responsible for elimination of transduced cells in vivo by effector mechanisms involving Fas-FasL and perforin-granzymes. TH2 cells on the other hand secrete IL-4, which helps B cells differentiate into antibody secreting plasma cells. Secretion of neutralizing antibodies results in blocking of vector readministration. The nature of the neutralizing antibody response can be measured by determining the antigen (by Western blot) and isotype of the immunoglobulin.

Understanding the molecular mechanisms involved in the cascade of events from antigen uptake by antigen presenting cells to differentiation of T and B cells, will allow development of therapeutic immunosuppressive agents to allow persistent transgene expression and ability to readminister viral vectors.



Induced Pluripotent Stem Cell Core Facility (Penn)

Summary:

The Penn Institute for Regenerative Medicine (IRM), which has been at the forefront of stem cell research and translational medicine, established the iPSC Core in 2009 to promote this powerful technology on campus and surrounding institutions. The goals of the Core are:

• to facilitate derivation of induced pluripotent stem (iPS) cells from somatic cells;
• to provide expertise and training to researchers in embryonic stem (ES)/iPS cell culture;
• and to serve as a resource for sharing iPS cell lines and iPSC technology within the UPenn and the broader scientific community.



Interventional Radiology Animal Catheter Lab (Penn)

Summary:

In the Interventional Radiology Catheter Lab minimally invasive procedures are performed via fluoroscopy, ultrasound and endoscopy. Percutaneous vein and arterial access is performed via ultrasound-guided technique. Surgeons are then able to guide catheters, ballon dilation, and other small instrumentation through the blood vessels. Procedures that have been performed in the lab are:

• Angiography
• Aneurysm creation
• Balloon angioplasty
• Embolization (coil, glue, embospheres)
• Inferior vena Cava (IVC) filter placement & retrieval
• Selective arterial catheterization
• Stent placement (renal, gastric, iliac)
• Peroral gastroenteric anastomosis

This lab has capabilities for full surgical and anesthesia protocols and full fluoroscopy imaging. Included in the lab are a small office space, an LCD monitor and computer for the fluoroscopy unit, eye wash station and a surgeon scrub sink.



Investigational Drug Service (Penn)

Summary:

The Investigational Drug Service (IDS) is the research pharmacy for the University of Pennsylvania, providing services for clinical and pre-clinical drug and device trials to investigators at all Penn schools, UP Health System hospitals and clinics and affiliated institutions.

The IDS is able to offer a range of services to investigators, from preparation, dispensing and inventory management for inpatient and outpatient trials, to formulation of blinded dosage forms or placebos to match existing medications, randomization tables and blinding schemes, specialized packaging and distribution, as well as limited release testing of finished products. We can assemble draft materials for CMC (IND) submissions or draft language for protocols, describing our activities related to your specific protocol. As a core facility, we pass along our actual costs on an hourly basis for these services. We maintain a highly secure, temperature-controlled facility and an electronic inventory system.



Islet Cell Biology Core (Penn)

Summary:

General Mission Statement:
To serve as a resource for Center Investigators that are studying pancreatic islet cell biology by offering islet isolation and culture, functional phenotyping of islets and providing consultation and help to develop strategies how to use the services of the core optimally or attempt to modify available technologies to solve particular problems.

Researcher's Note:
Please remember to acknowledge the Institute for Diabetes, Obesity and Cardiovascular Metabolism, the DRC grant (P30DK19525) and the services of the Islet Cell Biology Core in any ensuing research publications.



Laser Confocal Microscope Core (Penn)

Summary:

Penn Dental Medicine houses a Radiance 2100 laser confocal microscope available for use by researchers throughout the School as well as others inside and outside the University.



Mass Spectrometry Facility (Penn)

Summary:

"The Mass Spectrometry Facility is part of the Shared Instrument Facilities of the Department of Chemistry. It provides low and high resolution mass spectra to Penn Chemistry and to other research groups throughout the university community for the determination of elemental composition and purity of a wide variety of compounds."

Investigators interested in using the facility are encouraged to call and discuss their project. Usage fees will be quoted at this time. Fees depend upon the instrument involved in the analysis, complexity of the project, training, etc.



Mass Spectrometry Molecular Profiling Core (Penn)

Summary:

This core provides sophisticated analytical services based on liquid chromatography-mass spectrometry.



Metabolic Tracer Resource (Penn)

Summary:

The Metabolic Tracer Resource aims to provide consultation and services to IDOM investigators interested in using stable isotope labeled tracers (typically carbon-13 or deuterium) in cell-based, animal and human metabolic studies.

The Resource offers analysis of stable isotope enrichment of glucose, glycerol, fatty acids and amino acids in samples from metabolic tracer studies. These data can then be used to calculate rates of turnover, synthesis, production or recycling.

The Resource is currently located in room 12-171A Translational Research Center (TRC).



Microbial Culture and Metabolomics Core (Penn/CHOP)

Summary:

The Microbial Culture and Metabolomics Core features facilities and equipment for the aerobic and anaerobic culture of microbial species in both batch and continuous systems as well as services for targeted metabolomics. The core offers training and usage for all of these equipment as well as consultation towards experimental design and method development of microbial culture studies. Additionally, the core offers anaerobic culture services; working with the researcher, the core will purchase, receive, and revive strains from commercial culture collections (i.e., ATCC, DSMZ). The core will prepare glycerol stocks, liquid cultures, or gavage-ready suspensions for inoculation of animals with pure or define-mixed microbial communities.



Microbiome Human Intervention Core (Penn/CHOP)

Summary:

The Human Intervention Core offers a wide array of services to assist with the design and implementation of microbiome studies. The core can assist with longitudinal studies as well as pilot studies. Pilot studies can be rapidly implemented with human intervention core staff, project managers and research coordinators, to conduct these studies."

"Please fill out this form and send to: nessel@mail.med.upenn.edu and uroy@mail.med.upenn.edu



Microscopy Core (Penn)



Mixed Methods Research Lab (Penn)

Summary:

"The goal of the Mixed Methods Research Lab (MMRL) in the Department of Family Medicine and Community Health is to foster the use of qualitative and mixed methods research methodologies with a focus on integrating key stakeholder perspectives and goals into research designs. The MMRL works with investigators to provide conceptual and technical support for community based and clinical research questions. Qualitative, mixed methods and action research are uniquely suited to capture the contextual, socio-cultural, and experiential factors that contribute to health disparities.

The MMRL offers consultation, training, and staff support at all stages of the research process, including project and proposal conception, instrument development, budget development, data collection, data management, analysis, and publication/dissemination."

MMRL staff has expertise in a variety of traditional and innovative data collection methods including observation, freelisting, individual interviews, and focus groups. The MMRL primarily uses a modified grounded theory approach to analyzing data. Grounded theory is a methodology that involves iterative development of theories about what is occurring in the data as they are collected. The process develops themes that emerge “from the ground,” based on responses to the open-ended questions developed for the proposed study.



Molecular Profiling Facility (Penn)

Summary:

The Penn Molecular Profiling Facility provides instrumentation and expertise for DNA and RNA profiling. Microarrays and other highly parallel technologies provide the means for measuring the identity and abundance of DNA and RNA for targeted genes, or the whole genome, in a biological sample. The Facility offers a range of cost and performance options suitable for a variety of experimental questions. Molecular assays are critical to many aspects of basic, clinical, and population research, including molecular stratification of patients entering clinical protocols, molecular epidemiological and pharmacogenetic studies, as well as longitudinal follow-up of patients in clinical investigations.

Since molecular technologies and instrumentation are evolving rapidly, the centralization of molecular testing services within this core facilitates utilization of leading-edge molecular analyses by the investigators. Because some assays are used for clinical decisions during clinical trials, tests are meticulously designed and performed with strict attention to the prevention of polymerase chain reaction (PCR) contamination.

The Facility is a fully equipped molecular biology laboratory staffed by experienced individuals in developing and performing molecular biological assays. While the Facility staff performs most of the assays, investigator-performed studies are actively encouraged through the sharing of Facility procedures, individualized training of investigators or their technical staff, and use of core equipment.
The Facility Director and the Technical Director are available to talk with investigators to explore how the services of the facility can enhance or design their specific research projects. We invite investigators to meet with us in the planning stages of their studies, especially before grant submissions, to discuss services that the core can provide, such as budget information and a description of the core for the resources section of the grant, as well as to plan collection and handling of the specimens for the study. The Facility is happy to custom design assays to fit an investigator's needs.



Molecular Profiling Facility, Bioinformatics (Penn)

Summary:

The bioinformatics staff of the Penn Genomic Analysis Core is available to provide experimental design and analyical services to the basic and biomedical research community. Our services include support for Next-Gen Sequencing data as well as all platforms available in the core. We provide services as one-on-one meetings with customized approaches determined by the experimental design and goals of the investigator. We translate experimental goals into statistical, analytical and visual prioritization of genes and pathways.



Molecular Screening Facility (Wistar)

Summary:

The Wistar Molecular Screening Facility is a shared resource facility accessible to Wistar and non-Wistar scientists. The mission of the facility is to enable investigators to 1) apply cutting edge technology and unique resources to discover molecular, genetic, and small molecule compounds suitable to further study the functions of poorly understood proteins, signaling pathways, and cells in complex biological processes relevant to human physiology and disease; 2) foster collaborations; and 3) fulfill the long-term translational goal of the Wistar Cancer Center of merging basic mechanisms of cancer biology with disease-relevant themes of early-phase drug discovery and new target identification.

The Shared Resource operates on a fee-for-service basis, providing expertise in bridging automated technology with the development of innovative assays for high-throughput chemical and functional genomic screens. The laboratory strives to possess the flexibility to accommodate diverse biological systems and a variety of investigator-developed assay types. While service is the primary role of the laboratory, the staff will also develop and implement new technology as needed to fulfill the needs of its users. Education and training is also part of the laboratory's mission, as trainees apply high-throughput screening experiments to their investigations. The combinations of these activities will provide scientists opportunities to develop new innovative basic and translational research, preliminary data for hypothesis driven research grant applications, and public-private partnerships.

The Wistar Molecular Screening Facility was developed with support from the Commonwealth of Pennsylvania Department of Community and Economic Development Keystone Innovation Zone initiative, The F. M. Kirby Foundation, The CLAWS Foundation, The Florence & Daniel Green Foundation, The McClean Contributionship, From The Heart Foundation, the Noreen O’Neill Foundation for Melanoma Research, NIH shared instrumentation grants, and an NCI Cancer Center Support Grant.



Mouse Cardiovascular Physiology and Microsurgery Core (Penn)

Summary:

The Mouse Cardiovascular Physiology Core Facility, established in April 2006, is under the leadership of Dr. Tao Wang. Our goal is to aid investigators with the creation of mouse models related to cardiovascular diseases. We provide comprehensive assessments of cardiovascular structure and function using high-resolution echocardiographic imaging tools, which provides a unique and valuable research opportunity for translational research from animal studies to human applications. We deliver on-demand services that reveal important cardiovascular, structural, and functional aspects of mouse models in an efficient and cost-effective manner.

We offer a variety of surgical procedures, mouse models of cardiovascular disease, invasive hemodynamical measurements of pressure and ventricle function, high-resolution ultrasound imaging, and ECG/BP telemetry services for the comprehensive evaluation of cardiovascular phenotypes of transgenic and knockout mice. We perform surgical procedures that may be difficult or time-consuming for investigators without the proper expertise, experience, and/or equipment. Furthermore, we provide surgical training for various mouse models and data analysis for physiological assessment of cardiovascular function.



Mouse Phenotyping, Physiology and Metabolism Core (Penn)

Summary:

Core Aims:

To offer expertise and services for determining the metabolic and endocrine phenotpyes of mice with diabetes, obesity and related disorders.

To create a database of physiologic, endocrine and metabolic measurements in mice.



NBIC Probe Facility (Penn)

Summary:

The NBIC serves as an incubator for new probes of nanostructure behavior and associated instrumentation development. It is equipped with a suite of scanning probes, opto-electronic/transport tools, and optical probes that are so recently developed as not to be available on commercial instruments. The environment facilitates the development and refinement of new probe-based techniques.



Neurobehavior Testing Core (Penn)

Summary:

Although there have been major advances in Psychiatry and Neurology, less progress has been made toward understanding nervous system function, specifically the mechanisms underlying behavior. The Penn Medicine Neuroscience Center (PMNC), the Institute for Translational Medicine and Therapeutics (ITMAT), the Center for Sleep and Circadian Neurobiology (CSCN) and the Perelman School of Medicine(PSOM) established the Neurobehavior Testing Core (NTC) for behavioral phenotyping of mice. In addition to serving as a resource for neuroscience researchers, the NTC can be utilized by scientists in other disciplines who are interested in the behavioral consequences of other physiological (e.g., metabolic) disruption.

The NTC offers comprehensive testing of mouse models of disease and experimental compounds. Investigators can select from a broad range of assays that can be tailored to their specific interests. Our assays include tests for Learning and Memory, Circadian and home-cage activity monitoring, Affective disorder-related behaviors, Social interaction, Sensory and Motor function, Drug addiction-related behaviors and electrophysiological recording. The NTC also provides consultation, assistance in writing protocols and data analysis. The NTC can train personnel from an investigator’s lab to perform experiments at a reduced cost. Please contact Dr. W. “Tim” O’Brien at obrienw@mail.med.upenn.edu or (215) 898-0476 to discuss how the Neurobehavior Testing Core could facilitate your research.



Neurons R Us (Penn)

Summary:

Neurons R Us is a service center provided by the Mahoney Institute of Neurological Sciences at the University of Pennsylvania. We have been supplying neurons for research to the Penn community for over 25 years. The center's Technical Director, Margie Maronski, won Penn's 2008 Models of Excellence award for her work providing outstanding cultures to Penn researchers.

Advantages of buying from us
• Low cost
• On campus
• Mouse and rat
• Hippocampus and neocortex - other tissues upon request
• Healthy, longer lasting cultures
• Provided in dishes, wells or in suspension; with or without glia
• Expert advice and troubleshooting
• "Made to order" cultures upon request, e.g. transgenics, other strains



Next-Generation Sequencing Core (Penn)

Summary:

The NGSC offers ultra high throughput sequencing services for the PSOM research community. We offer library quality assessments, sequencing, and optional preliminary data analysis for a wide variety of experimental protocols including ChIP-seq, RNA-Seq, HITS-CLIP, miR-Seq, exome capture, and BIS-seq. We offer limited library preparation services, but can advise on library preparation techniques. We have two Illumina hiSeq2000s for large-scale sequencing and a MiSeq for sample evaluation or library testing. To get started, visit our website, create an account for yourself, then create a new experiment and we will contact you.



Nucleic Acid/Protein Core (CHOP)

Summary:

The Nucleic Acid/Protein Research Core Facility, located on the 9th floor of the Abramson Building, provides a centralized source for specialized services, technical expertise and reagents to support investigators' molecular biology research needs. These services include DNA sequencing analysis, fluorescent fragment analysis (microsatellite genotyping), oligonucleotide synthesis, microarray services, denaturing high-performance liquid chromatography, Affymetrix GeneChip expression analysis, SNP analysis and RNA analysis.



Ocular Viral Vector Core (Penn)

Summary:

The CAROT Research Vector Core is a facility that specializes in generating recombinant Adeno-Associated Virus (AAV) vectors for applications in retinal and ophthalmic research. The main objective of the Core is to provide custom-made vectors for basic and translational research. The Core will guide investigators on selection of capsids, regulatory elements and other issues that may impact the results. The core can scale the size of the vector preparation according to the needs of the investigator. All vector lots undergo evaluation to assure purity and high quality. Dr. Shangzhen Zhou an internationally recognized leader in AAV vector production directs the core.



Ocular iPS Cell Core (Penn)

Summary:

THE CAROT iPS Cell Core is focused on creating a biorepository of cells from individuals with inherited retinal degenerations and on using cells from the repository to establish induced pluripotent stem cell lines that can be differentiated into ocular- and retinal- specific lineages. The use of patient derived iPSC allows the Core to create disease- and patient- specific personalized models of disease. A primary objective of the Core is to use patient iPSC-derived cell models for proof of concept studies and drug screening for new therapeutics. Additionally, the Core will provide training and guide investigators in the creation of iPSC lines and their differentiation into various retinal specific lineages. The Core can also provide liquid nitrogen storage of derived cells. The Core Director, Dr. Jeannette Bennicelli, is a cellular and molecular biologist with expertise in the derivation and manipulation of cell lines as well as design, construction, and testing of therapeutic AAV vectors.



Outcomes Measurement Methods Core (Penn)

Summary:

Mission:
The mission of the Outcomes Measurement Methods Core Program is to provide investigators, key personnel, and trainees with research collaboration, education, and consultation in the selection and development of measurement tools for translational and clinical research projects.

Initial consultations are provided free of charge. Cost recovery is required for follow-up consultations or long-term collaboration.

The OMMC is managed by the Center for Health Behavior Research (CHBR).



Pathology Clinical Service Center (Penn)

Summary:

The mission of the Pathology Clinical Service Center (PCSC) is to promote and facilitate translational research by providing comprehensive blood and tissue-based services to investigators. Among these services are those that traditionally are only provided by Anatomic and Clinical Pathologists in the clinical setting. The Anatomic Division (PCSC-AP) specializes in the analysis of human bio-samples and offers histology, immunohistochemistry, immunofluorescence, in situ hybridization, tissue microarray construction, molecular analysis, digital imaging, multispectral image analysis and assay development. The Transfusion Medicine & Therapeutic Pathology Division (PSCS-TM&TP) encompasses the Apheresis and Infusion Clinic, the Stem Cell Lab, and the Blood Bank, and the Penn Medicine Blood Donation Center. The PCSC-TM&TP operates in compliance with FDA regulations, is accredited by the AABB and FACT, and specializes in the collection, processing, and re-infusion of cellular products, and offering mononuclear cell, whole blood, and plasma collections, elutriation, and infusion of intravenous medications under medical supervision. The Clinical Pathology Division (PCSC-CP) specializes in the analysis of blood and serum samples, including chemistry, microbiology, coagulation, hematology, immunology, and molecular pathology. In addition, PCSC can collect, store, analyze, and annotate research samples for IRB-approved projects.



Pathology Core Laboratories (CHOP)

Summary:

The Pathology Core Laboratory at the Research Institute at Children's Hospital of Philadelphia unites several core pathology components in one facility. Path Core provides basic histopathology, research immunohistochemistry, tissue microarray, and laser capture microdissection services to researchers at Children's Hospital of Philadelphia and within the surrounding academic community. We offer a full range of histopathology services for both paraffin-embedded and frozen tissue samples including tissue processing, embedding, and cutting. We also perform most standard stains as well as immunohistochemistry, antibody workup, fluorescence, in situ hybridization and TUNEL. Tissue microarrays can be constructed and our staining services may be used on slides acquired from the arrays. Sophisticated imaging instrumentation is available for both bright field and fluorescent microscopy including whole slide scanning. We also host specialized software to analyze, manage, and store data on stained tissues and arrays.



Penn Chemistry NMR Facility (Penn)

Summary:

Penn Chemistry NMR Facility provides researchers in the Chemistry and Materials Science and Engineering department access to state-of-the-art instrumentation for high resolution NMR spectroscopy. The Facility provides users extensive training to use spectrometers without supervision and expert advice/consultation on advanced applications of NMR spectroscopy to solve research problems.

At present, the Facility operates ten high resolution NMR spectrometers (300-600 MHz) of varying capabilities located in the Chemistry building at the corner of 34th and Spruce street, Philadelphia, PA."

"Penn Chemistry NMR Facility provides limited solution NMR services (data acquisition and spectrum/structure analysis) to other departments/centers of University of Pennsylvania based on hourly charges. However, these services will be limited to the availability of instrument time and personnel.

NMR Facility accepts service samples from outside academic and industrial customers based on per hour charges.



Penn Gene Targeting Core and Laboratory (Penn)

Summary:

The Penn Gene Targeting Core and Laboratory provides a truly complete knockout and knockin mouse service. In addition it provides all steps necessary for generation of knockout and knockin mutations in human embryonic stem cells (human ES cells) and human induced pluripotent stem cells (human IPS cells). For overview see Main Services below and for details see here. Depending on each project, PGT is using classical gene targeting methods or the revolutionary CRISPR/Cas9 – based system or a combination of both:

• targeting vector design and construction
• CRISPR/Cas9 design and construction
• electroporation of the targeting vectors and/or CRISPR/Cas9 guide RNAs into mouse ES cells or human ES or IPS cells
• PCR/Southern genotyping and karyotyping of the resulting ES/IPS clones
• targeted mouse ES cell injection into blastocysts (usually together with the Transgenic and Chimeric Mouse Facility)

It also provides general genomic Southern/PCR services for genotyping of mouse tails as well as any cell type from which sufficient genomic DNA can be obtained (human ES cells, human iPS cells, mesenchymal stem cells and others).



Penn Gnotobiotic Mouse Facility (Penn)

Summary:

The Penn Gnotobiotic Mouse Facility (PGMF) provides centralized germ-free and gnotobiotic mouse services. The PGMF maintains several common strains of germ-free mice that are available upon request, and provides re-derivation services for generating customized germ-free and gnotobiotic mouse strains. In addition, the PGMF offers the Penn research community access to isolators for utilizing germ-free and gnotobiotic mice during IACUC-approved experimental procedures. To further meet the needs of investigators, the PGMF provides technical support required for various experimental procedures.



Penn Medicine Academic Computing Services (Penn)

Summary:

The Penn Medicine Academic Computing Services (PMACS) organization was recently formed through the consolidation of several of the largest groups on campus providing computing services to departments, centers and institutes. The PMACS team now consists of approximately eighty information technology professionals providing services such as desktop support, server administration, storage management, high performance computing, software development, data base development, vendor application deployment/support and staff leadership. This new organization will continue to evolve and grow to meet the education, research and administrative computing needs of the entire Perelman School of Medicine.



Positron Emission Tomography Center (Penn)

Summary:

The PET Center is dedicated to continuing the advancement of molecular imaging and seeks to build a network of collaborators to conduct translational research using existing and new radiotracers to help better understand the diagnosis, physiology and treatment of multiple diseases.

We strive to educate referring clinicians and their patients about the emerging benefits of PET/CT diagnostic procedures, other radiotracer imaging methods and radionuclide therapies as tools in their research and clinical practice.

The PET Center is committed to providing opportunities and mentoring for individuals interested in pursuing work or collaborations within the molecular imaging field.



Protein Expression Facility (Wistar)

Summary:

The Protein Expression Facility is a shared resource laboratory that provides Wistar Cancer Center Members and non-Wistar scientists technical assistance with viral vector preparation and the expression and purification of recombinant proteins. The Facility has greater than 20 years of experience in recombinant protein expression with special expertise in the use of baculovirus expression systems (BVES). The Facility offers the following services:

1. Recombinant plasmid DNA engineering
2. Viral vector production (i.e. baculovirus and retrovirus)
3. Analytical and preparative scale expression of nascent or epitope-tagged recombinant proteins
4. Protein purification

These goals are accomplished by a centralized laboratory with dedicated, experienced staff, which enables high-throughput, economy of scale, virus preparation and protein expression services, including quality assurance and control procedures to ensure efficient, consistent production and purification of recombinant proteins and viral vectors. Many recombinant proteins produced by the facility have been used for crystallization efforts, analytical biochemistry studies designed to investigate enzymatic properties, structure-function relationships between protein-protein, protein-nucleic-acid, and protein-small molecule interactions, custom antibody production, experimental cancer vaccines, and development of miniaturized assays for small molecule screening.

The facility is supported in part by an NCI Cancer Center Support Grant and a grant from the NIH National Institute of Aging (PO1 AG031862).



Proteomics Core Facility (CHOP)

Summary:

The Protein Core Facility (PCF) at CHOP addresses the overwhelming need for the technological resources to identify, produce and characterize new proteins. The PCF has four operating modules available: Protein expression, Characterization, Protein-Protein Interactions, and Proteomics. While many of our services are routine, we are also committed to collaborating with the CHOP community to overcome difficult research obstacles. We strongly encourage CHOP researchers to bring their research needs to our attention, so that we can try and provide solutions.

PROTEIN EXPRESSION AND PURIFICATION: With the advent of high-throughput DNA sequencing technology and the identification of an increasing number of genes and ORF's, there is an increasing demand for fast and efficient protein production. While some proteins can be expressed in bacteria, other proteins require other expression systems. These might include proteins that require certain chaperones for folding, are toxic to bacteria, contain multiple disulfides, have glycosylation sites, or require other extensive post-translational modifications for their activity. The PCF provides protein production in insect cells (sf9, sf21) using the Baculovirus expression system. We also offer assistance with expression of bacterial proteins or in vitro translation. For purification of the recombinant proteins we offer affinity, ion exchange and size exclusion chromatography.

PROTEIN CHARACTERIZATION: In addition to offering expression services, the PCF provides analyses such as mass spectrometry, gel electrophoresis, analytical and/or preparative chromatography, spectroscopy (fluorescence, circular dichroism, ultraviolet-visible), ultracentrifugation, and phosphorimaging. We also offer assistance in refolding recombinant proteins.

PROTEIN-PROTEIN INTERACTION: The identification of interacting protein partners is fundamental to the understanding of signaling networks and drug-discovery. The PCF has a number of tools for identifying new protein-protein interactions. Options include the SELDI protein-chip analyzer, analytical ultra-centrifugation, and Surface Plasmon Resonance (Biacore). We are also implementing, in collaboration with Invitrogen, an new protein-interaction array service. Once new partners are detected, we offer protein identification using mass spectrometry. Please consult with Steven Seeholzer for more information regarding these techniques.

PROTEOMICS: The PCF is committed to providinging the latest technologies in mass-spectrometry, multi-dimensional chromatography, protein-arrays etc. Utilizing these tools will enable us to uncover more new protein sequences and protein-protein partners so that we might have a better understanding of proteins within specific networks and at the center of pediatric diseases. This knowledge will be critical if we are to successfully understand and treat medical problems that arise at the biochemical level.



Proteomics Facility (Wistar)

Summary:

The Wistar Proteomics Facility provides mass spectrometry (MS) and sequence analysis of proteins and peptides at maximum sensitivity using state-of-the-art instruments and methods.

The most commonly used services are identifications of either purified proteins or complex protein mixtures, such as sub-proteomes or complete proteomes, using electrospray ionization tandem mass spectrometry (ESI MS/MS). Typically, either individual bands are excised from 1-D SDS gels, or the entire gel lane is analyzed by slicing it into uniform fractions followed by trypsin digestion and nanocapillary HPLC interfaced directly with hybrid ion trap mass spectrometry (Gel/LC-MS/MS). Data is analyzed and filtered to produce low false-positive rates. Several options are available for quantitatively comparing protein changes in related samples, and additional options will be implemented in the future.

Complementary services include reverse-phase microbore HPLC peptide mapping with UV detection and mass measurements of intact peptides and proteins using MALDI MS or ESI MS. Posttranslational modification (PTM) analyses including identifications of specific modified residues also are provided, although investigators should recognize that in most cases these studies are quite complex and require substantial effort. These studies, as well as analyses of complex protein mixtures, usually require preparation of custom sequence databases and/or custom data analyses, which can be provided by the facility as needed.



Proteomics and Systems Biology Core (Penn)

Summary:

The Proteomics Core Facility in the Penn Genomics Institute is a service and collaborative research resource that balances applied proteomics research with the development of new and improved methods for protein identification, characterization, and quantification. The facility encourages collaborations that apply the tools of proteomics to cutting edge biomedical research.

The Proteomics Core Facility is a center not only for services but also for basic and collaborative research and development of two-dimensional gel electrophoretic - and mass spectrometric-based techniques. Prospective users are encouraged to make their inquiries either by e-mail (ian@spirit.gcrc.upenn.edu), or stop by our facility on the eighth floor of BRB II/III.



RADCORE (Penn)

Summary:

Today, our mission is to provide a support system for coordinators within the Department of Radiology and:

• Build a strong research coordinator team within the Department of Radiology
• Set up a network for research coordinators where assistance can be provided in the orientation of new research coordinators, and in the education of co-workers through the sharing of expertise (ex., MR, CT, and/or PET)
• Gain knowledge and further careers within the Department of Radiology
• RADCORE also houses an IND Support Service that manages a portfolio of regulatory support for investigational diagnostic imaging probes requiring IND or RDRC regulatory approval and support. This service is managed by Kathleen Thomas, whom investigators interested in investigational tracers should contact. See also PET Center for further information about investigational PET imaging probes.



Radioimmunoassay and Biomarkers Core (Penn)

Summary:

"The assay service has proven essential for current research in the DRC and Penn community. The core used to focus mainly on rat and human insulin, glucagon and C-peptide to a diverse, high-volume and cost-effective service. Over 50 different diabetes and endocrinology-related markers can be assayed.

In conjunction with the Clinical and Translational Research Center (CTRC), we are now offering multiple analyte (multiplex) services. As part of a collaborative initiative, Dr. Collins initiated and established Luminex IS100 multiplex ELISA services. This system uses cell-sorting technology to measure multiple proteins simultaneously. This technology will be of great use for investigators studying transgenic mice, where sample volumes are low. In addition, the multiplex platform allows for screening of human cohorts in disease research particularly of small volume in repeated sampling protocols.

The objectives of the Radioimmunoassay and Biomarker Core includes:

• delivery of new services to existing and new investigators
• development of informatics infrastructure for efficient delivery of service
• engage proactively in outreach to the DRC/PENN research community to enhance efficiency, cost-sharing and increase users of Biomarker Core services."

The assay services listed are not an exhaustive list but rather a list of assay services we have provided in the past. Please contact the RIA/Biomarkers core if you would like to assay for an analyte that is not listed. We can help you find the appropriate kit and provide the assay service.



Recruitment, Outcomes, and Assessment Resource (Penn)

Summary:

The goals of the Recruitment, Outcomes and Assessment Resource (ROAR) are to develop resources for population and clinical/transitional research that can enhance collaborative, multidisciplinary population research; enable observational, behavioral, clinical translation and interventional studies; and avoid inefficiency in the development and execution of these studies.

The ROAR, which is led by Dr. Karen Glanz, is comprised of two coordinated components: one for Recruitment, Retention and Outreach and a second for Research Implementation.



Regulatory and Clinical Trial Site Services (Penn)

Summary:

CAROT is an integral part of the Scheie Eye Institute, one of the oldest and most reputable ophthalmology clinics in the USA. This institute has an established history of successfully executing clinical trials using a diverse patient population.

CAROT offers comprehensive testing facilities to complete most study protocols in compliance with several health authority standards. As a pioneer in the field of gene and cell therapy, CAROT contains unique endpoint measures such as mobility tests, functional magnetic resonance imaging (fMRI) and adaptive optics scanning laser ophthalmoscopy.

CAROT also manages clinical, regulatory and trial site services to guide investigators through the clinical and market authorization process, with speed, accuracy and safety.



Research Ethics Program Core (Penn/CHOP)

Summary:

The mission of the Research Ethics Program is to provide investigators, key personnel, and trainees with research collaboration, education, and consultation that address ethical issues in the design and conduct of translational and clinical research. We are primarily engaged in contributing to the CTSA's research ethics educational needs, and we provide consultation services for CTSA and other researchers facing ethical issues in planning or performing their studies. We also have research interests in identifying new and emerging ethical issues in translational research and are looking for collaborative opportunities to better understand this research paradigm.



Research Instrumentation Shop (Penn)

Summary:

The Research Instrumentation Shop is non-profit, shared resource machine shop of the University of Pennsylvania, Perelman School of Medicine. Its mission is to assist University faculty in the design and construction of both laboratory and clinical instrumentation. The staff is comprised of mechanical and optical specialists and is experienced with working with scientists to design and construct custom research clinical instrumentation and apparatus.



Singh Center for Nanotechnology (Penn)

Summary:

The Singh Center is centered around four major research facilities, all featuring state-of-the-art equipment for nanoscale characterization, measurement, and fabrication: the Quattrone Nanofabrication Facility, the Nanoscale Characterization Facility, the Scanning and Local Probe Facility, and the Material Property Measurement Facility.

The following connected sections comprise the major components of the building:

• A 10,000 square-foot next-generation Cleanroom Facility for micro/nanofabrication, including tooling for nanoscale and soft materials integration and a novel nano/bio bay serves as the home of the Quattrone Nanofabrication Facility.

• A 10,000 square-foot advanced underground facility designed for temperature stability and excellent isolation from vibrational, acoustic, and electromagnetic noise serves as home of both the Nanoscale Characterization Facility and the Scanning and Local Probe Facility.

• A Property Measurement Facility provides state-of-the-art measurement capabilities in magnetometry, optics, electrical and thermal transport.

• 18,000 net square feet of space for other shared facilities and general laboratories housed in an adjoining three story structure; a glass-enclosed galleria with views into the cleanroom; conference rooms; and a forum for meetings.

The building houses a large suite of high-performance equipment for nanotechnology research, including electron and scanning probe microscopy, cleanroom tools, electron beam lithography, and several materials synthesis and characterization instruments.

The multi-user facilities are vital to the research and educational programs at Penn and are leveraged by partner institutions and local industry within the Mid-Atlantic region. Unifying these central resources fosters the exchange of scientific ideas and the development of nanoscale science and technology, brings together crosscutting capabilities and the staffing to support these tools, and provides the modern infrastructure necessary to establish a regional center for nanotechnology.



Small Animal Imaging Facility (Penn)

Summary:

The SAIF combines state-of-the-art instrumentation and a nationally recognized staff to assist investigators with a wide range of imaging based experimental approaches. The SAIF currently provides a comprehensive suite of imaging modalities including:
• Magnetic resonance imaging (MRI) and spectroscopy (MRS)
• Optical imaging (including bioluminescence, fluorescence, and near-infrared imaging)
• Computed tomography (CT)
• Positron emission tomography (PET)
• Single photon emission computed tomography (SPECT)
• Ultrasound (US)

In addition, dedicated housing is available for mice and rats undergoing longitudinal imaging studies. Ancillary facilities and resources of the SAIF are devoted to chemistry, radiochemistry, image analysis and animal tumor models, including assistance with animal handling.



Small Animal Imaging Facility: MRI/MRS Sub-Core (Penn)

Summary:

These studies are performed on a wide range of biological samples including small animals (cats, rabbits, rats, mice), tissue specimens, cultured cells and tissue extracts.

This facility includes a conveniently located, well equipped surgery room used for preparing the animals for MR exams and a wide assortment of supporting equipment, i.e. anesthesia machines, MR compatible vital signs monitors (SA Instruments), infusion pumps (Harvard), heating pads, etc. A variety of perishable supplies used in animal preparation are also provided by the facility.



Small Animal Imaging Facility: Nuclear Medicine Sub-Core (Penn)

Summary:

The PET Center operates various PET, SPECT, and CT scanners for different research and scanning needs.



Small Animal Imaging Facility: Optical/Bioluminescence Sub-Core (Penn)

Summary:

The Optical/Bioluminescence Sub-Core of the SAIF provides the capability to perform cellular and molecular non-invasive in-vivo bioluminescence, near-infraredfluorescence and Cerenkov imaging.

The instrumentation allows sensitive, non-invasive molecular imaging for a variety of applications including detection and quantification of various bioluminescent or fluorescent reporter-expressing cells or tissues (in culture or in small animals).

The facility currently houses a Perkin Elmer IVIS Lumina II, two LI-COR Pearl Impulse Imagers and two Perkin Elmer IVIS Spectrums. The Facility offers assistance with experimental design, regulatory approval, troubleshooting, data management, analysis and display.



Small Animal Imaging Facility: Ultrasound Sub-Core (Penn)

Summary:

The Ultrasound Sub-Core of the SAIF offers an array of research services for pre-clinical research including quantitative image analysis and consultation.

Our state-of-the-art ultrasound scanners are available as a resource for conducting your research studies. This rich resource for ultrasound imaging is available at nominal hourly fees for various categories of study.

Ultrasound Research Services provides services to a host of groups working on diverse projects such as the measurement of angiogenesis, vascularity, tissue elasticity, the effects of various pharmaceuticals on these measures and more. Such research encompasses a variety of clinical areas including radiology, oncology, cardiology, gynecology, and hematology, among others.



Stem Cell & Xenograft Core (Penn)

Summary:

The Stem Cell and Xenograft Core (SCXC) is a comprehensive resource laboratory that integrates a viable tissue bank of normal human hematopoietic cells and hematopoietic malignancies with a full range of xenograft services.

The SCXC is committed to facilitating and promoting translational research involving viable primary human hematopoietic tissues. Our core offers adult whole bone marrow from healthy donors and umbilical cord blood. Mononuclear and CD34+ cells from normal bone marrow and cord blood are available, and other cell fractions can be provided by arrangement. We maintain a large tissue bank of cells from hematopoietic malignancies including AML, ALL, CML, MDS and MPDs. All samples are fully annotated and frozen as viable cells. We also offer access to an immunomagnetic cell sorter (Miltneyi AutoMacs). Expertise in primary human hematopoietic stem/progenitor and leukemic cell culture and manipulation is available. For consultation or questions, please contact Martin Carroll.

The SCXC offers a wide variety of xenograft services from training to full-service experiments. The Core maintains a large breeding colony of immune-deficient (NSG) mice for users xenograft studies. We also offer human immune system (CD34-transplanted) NSG mice for a wide variety of studies ranging from gene therapy to HIV. Experimental animals are housed in dedicated BSL-2 animal barrier space equipped for whole body irradiation and all necessary procedures and survival surgeries. Currently established xenograft models include normal human CD34 and leukemia engraftment, human iPS and ES-derived teratomas, human skin grafting, orthotopic human ovarian, hepatic and pancreatic tumor cell injections, renal capsule implantation. We also offer access to a dedicated optical/fluorescence (IVIS Spectrum) imaging system located within the Core's BSL-2 space. For consultation or questions, please contact Gwenn Danet-Desnoyers.

Pennkey required to request services, contact to acquire a guest Pennkey if needed.



The Genotyping and DNA/RNA Analysis Core (Monell)

Summary:

This Research Core provides training and research support in genotyping and quantification of nucleic acids. The Core facility has equipment needed to genotype DNA samples and to measure nucleic acid concentrations in isolation or in tissue samples.



Transgenic and Chimeric Mouse Facility (Penn)

Summary:

The purpose of the Transgenic & Chimeric Mouse Facility is to provide a centralized service to efficiently produce transgenic mice for basic research. This should result in reduction in effort and cost to participating investigators. The facility is located on the basement level of the Clinical Research Building. This facility consists of an animal room and several injection rooms. The injection rooms are fully equipped to carry out the entire procedure of making transgenic mice. The animal room provides housing and breeding space for the mice involved in the transgenic projects. The facility uses sterile food and water as well as autoclaved cages and bedding; all cages are of the microisolator type to limit the spread of colony infection. The entire facility is located behind a microbiologic barrier where admittance is strictly limited and all personnel must wear sterile coveralls, gloves, hats, masks, and boots.



Translational Biomarker Core (Penn)

Summary:

This core provides sophisticated analytical services based on liquid chromatography-mass spectrometry.



Ultrasound Research Laboratory (Penn)

Summary:

The goals of the research laboratory are:

• To develop new ultrasound technologies and clinical applications
• To bridge the gap between technology and clinical applications
• To provide ultrasound imaging resources to other research groups within the Penn community and in other institutions

The laboratory consists of a core group of scientists, sonographers and technicians with expertise in ultrasound technology and computer programming. This group works with clinicians in multiple specialties; including radiologists, cardiologists and surgeons. Ultrasound Research Services, an arm of the laboratory, furnishes a state-of-the-art ultrasound scanner dedicated to research and serves the research community. There is a full-time sonographer and a part-time radiologist on staff to conduct clinical and pre-clinical imaging.

The research laboratory has been a valuable resource to several groups working on diverse projects. These include studies involving the measurement of angiogenesis, vascularity, tissue elasticity, contrast agents, and the effects of various physical and pharmaceutical agents on blood flow and tissue vascularity. The studies span a range of clinical areas including research on cancer, cardiovascular disease and musculoskeletal disease.



Vector Core (Penn)

Summary:

The Penn Vector Core is a full service viral vector core facility located on the University of Pennsylvania campus. With over a decade of experience in the production of viral-based vectors, the Core has become an important technological resource for investigators, both within and external to Penn, interested in the use of viral based vectors for gene transfer. The main objective of the Core is to provide investigators access to state-of-the-art vector technology for preclinical studies and other basic research applications. Such studies, utilizing carefully designed viral vectors, can provide information critical to the understanding of gene function and development of therapeutic vectors.

The Penn Vector Core specializes in the provision of novel AAV serotype vectors and has the greatest experience in producing novel serotype vectors developed at Penn. AAV1, 7, 8, 9 and rh10 were originally isolated at Penn in the laboratory of Dr. James M. Wilson and first made available to investigators through the Penn Vector Core. Due to its close proximity to the Wilson laboratory, the Penn Vector Core is able to rapidly assimilate new vector technologies and make them available to its users. The Core offers a variety of novel serotype AAV vectors and additional vectors currently under development will be distributed through the Penn Vector. All of the vectors generated by the Penn Vector Core are distributed under material transfer agreement (MTA) to academic, government and non-profit institutions. Corporate users may access novel AAV vector technologies through the Penn co-founded company, REGENXBIO Inc.



Viral Vector Clinical Core (CHOP)

Summary:

The Center for Cellular and Molecular Therapeutics at The Children's Hospital of Philadelphia has established a state-of-the-art cGMP clinical vector manufacturing suite for both adeno-associated viral vectors and Lenti viral vectors, help to realize the enormous promise of gene transfer therapy to address unmet medical needs.

The Core Facility utilizes a patented vector production technology and a highly efficient purification process that utilizes combined column and gradient centrifugation-based process steps. This system has manufactured clinical grade AAV vectors that have demonstrated excellent safety in several clinical studies.



Viral Vector Research Core (CHOP)

Summary:

Part of The Center for Cellular and Molecular Therapeutics, the Research Vector Core (RVC) has expanded its capacity to provide infrastructure support for investigators interested in using viral vectors in their research model systems. RVC utilizes molecular biology techniques to engineer and produce recombinant AAV vectors for gene transfer in research experiments or non-clinical studies. RVC staff work closely with investigators to plan and develop vectors for individual project requirements. The RVC has extensive experience in the production of multiple AAV serotypes. Due to its unique manufacturing method, resulting in high productivity, the RVC is able to deliver empty capsid free (<1%) vectors after the purification process. Reporter gene vectors are available directly from inventory. The core is under the direction of Dr. Shangzhen Zhou, a leading expert in AAV vector production. The core employs a team of dedicated technicians to provide research vectors with premium quality.



cGMP Lenti Vector Core (Penn)

Summary:

The CAROT Lenti Vector Core is a state-of-the-art production facility, with a Class 7 clean room suite. The production and QA/QC procedures meet the current GMP regulations as enforced by the FDA. Mr. William Chung is the director of the Lenti Vector Core GMP operations. The core group also provides CMC support for regulatory submissions with the assistance of Dr. Ilan McNamara.



hESC-iPSC (Stem Cell) Core (CHOP)

Summary:

The Stem Cell core provides expertise and quality-control reagents for the culture and differentiation of human embryonic stem cells (ESCs) and human induced pluripotent stem cells (iPSCs) to the CHOP and University of Pennsylvania academic communities. The facility maintains five of the new NIH-approved human ESC lines and several human iPSC lines. Stringent protocols are established to:
1. Propagate human ESC and iPSC lines, monitor for maintenance of the pluripotent state and distribute these lines to investigators.

2. Differentiate hESCs/iPSCs into mesoderm, endoderm and their derivative tissues under controlled, serum-free conditions with defined cytokines.

3. Generate iPSCs from human subjects.



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