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Clinical Cell and Vaccine Production Facility (Penn)

Director: Siegel, Donald L., PhD, MD


The Clinical Cell and Vaccine Production Facility (CVPF) renders bench-to-bedside translational medicine a reality. Equipped with state of the art facilities, the CVPF manufactures cell and gene biotherapeutics and is accredited by the Foundation for the Accreditation of Cellular Therapy (FACT). Further, the CVPF is the only GMP (good manufacturing practices) compliant facility on campus and functions as an NCI approved Abramson Cancer Center (ACC) Shared Resource. As an ACC Shared Resource and Path and BioResources core facility, the CVPF supports numerous investigational new drug (IND) protocols. Current protocols target a variety of disease indications (primarily HIV, adult and pediatric cancers, and stroke); many more trials are in development and, once approved, will further expand the scope of diseases targeted for cell and gene therapy. For more information on current trials, explore our “Clinical Trials” page.





  • Baxter CytoMate cell harvester ( Cell harvester )

  • Baxter Fenwal cell harvester ( Cell harvester )

  • Baxter Fenwal MaxSep magnetic cell separator ( Cell separator )

  • BD BACTEC 9050 blood culture system ( Blood analyzer )

    "The BACTEC blood culture system is a fully automated microbiology growth and detection system designed to detect microbial growth from blood specimens. The BACTEC™ FX and the BACTEC™ 9000 family of continuous monitoring blood culturing instruments offering performance, safety, reliability, ease of use, media quality and service. "

  • CaridianBCT Elutra cell separation system ( Cell harvester )

    "The Elutra Cell Separation System operates differently than conventional centrifugation-based cell separators that separate cells depending primarily on their density. Instead, the Elutra system uses counter-flow centrifugal elutriation to separate the different cell types."

  • COBE® 2991 cell processor ( Cell separator )

    "The COBE 2991 Cell Processor provides operational reliability using automation for control and consistency:

    • Variable spin times and speeds allow separation of components through centrifugation
    • The agitate mode washes desired cell components with a choice of solutions
    • Fluids or cells are expressed by the action of hydraulic fluid against a flexible membrane

    A reliable laboratory tool, the COBE 2991 Cell Processor includes:
    • Centrifuge unit with a flexible diaphragm
    • Hydraulic system
    • Operator panel with program board
    • 5 pinch valves
    • Red cell detector
    • Secondary control panel
    • Centrifuge cover interlock latch system

    Achieving results through automation:
    Automated cell processing with the COBE 2991 Cell Processor provides a high level of control for reproducible results:

    • Three operations per cycle (start/spin, supernatant-out, agitate/wash-in)
    • Precise programming of variable spin times adds automated accuracy and time-savings
    • Programmable memory stores up to 10 protocols, with the ability to make changes during procedures for increased flexibility
    • Three stop options: call the operator, or end the packed cells and re-suspended options

    Separating with centrifugal technology:
    • Separation of product components based on specific gravities
    • Removal and/or collection of desired fraction
    • Addition and agitation of wash solutions to remove unwanted cells, debris and fluids"

  • Coulter Multisizer 3 cell counter/sizer ( Automatic cytometer )

    "The Multisizer 3 provides highly accurate particle counting and sizing information utilizing the Coulter Principle."

  • FACScalibur flow cytometer ( Flow cytometer sorter )

  • Haemonetics Cell Saver 5 Autologous Blood Recovery System ( Cell separator )

  • Miltenyi CliniMACS® magnetic cell separator system ( Cell separator )

    "The CliniMACS® System offers a flexible platform for processing and separation of virtually any cell type as well as customized processing protocols according to specific sample requirements. Unique reagents and tubing sets also allow for the transition of promising research applications into innovative cell therapy concepts. "

  • Nova BioProfile 100 Plus Bioanalyzer ( Rapid multi-assay analyzer )

    The Nova BioProfile 100 Plus bioanalyzer is a fully automated cell culture analyzer that tests for gluatamine, glutamate, glucose, lactate, ammonium, pH, sodium, and potassium and calculates osmolality.


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Last updated: 2019-10-29T14:22:15.201-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016