The Histotechnology Shared Resource provides services for fixing, processing and paraffin or OCT-embedding of all types of tissues for light microscopy (e.g. routine stains, immunohistochemistry or in situ hybridization). The Facility staff performs routine hematoxylin, eosin staining, immunochemistry and FISH staining, as well as specialized staining and slide preparation for immunohistochemistry and in situ hybridization.
Frozen sectioning is also available, including consultation regarding freezing and fixing techniques to optimize experimental results.
Fixation, frozen tissue embedding and sectioning are available in the facility.
"Cutting frozen sections is sometimes best for doing immuno staining, especially when the antigen is masked by fixation. If frozen sections are required it is advisable to schedule an appointment.
Turnaround time for work submitted depends on the size of the project as well as the number of other projects that are being carried out at the time of submission. Often times the finished product can be obtained the next day and almost always within 3 days."
"Routine H&E (hematoxylin and eosin) staining is available at all times. Other special stains are done upon request. These include Trichrome for collagen, Bodians for nerve fibers, Luxol Fast Blue for myelin sheath, PAS for basement membrane and glycogen, Congo Red for amyloid, Oil Red O for fat and others that may be required for special projects."
Fixation (commercially bought 10% buffered formalin, Histochoice, Bouin's fixative, or specialty); decalcification (if the specimen contains bone); tissue processing and paraffin embedding; and sectioning (routinely at a thickness of 5 microns, but the thickness can be changed if requested) are available in the facility.