The objective of the Islet Cell Biology Core is to provide DRC members with state of the art support including experimental design, islet isolation, and performance of and/or training in an expansive range of assays for physiological and morphometric assessment of pancreatic islet function and growth. We also enlist unique expertise of newly interested faculty to adapt existing technologies to solve unique problems that cannot be addressed by standard methodologies. For example, new ties with our Physiology Department promise an expansion of consultation and services to study membrane biophysics critical for understanding normal and diseased islet cells.
Failure of insulin secreting pancreatic beta cells characterizes the progression of all forms of diabetes. The ICBC is thus positioned to contribute in a significant manner to the basic and translational research activities of the Institute of Diabetes, Obesity and Metabolism (IDOM) at the Perelman School of Medicine of the University of Pennsylvania. The ICBC has developed exceptional expertise in working with human and rodent pancreatic islet tissue, acquiring instrumentation and establishing procedures that are not readily available to the average laboratory.
"Investigating the behavior of free intracellular Ca2+ using state of the art fluorescence imaging techniques."
"Performing closed cell respirometry based on measuring islet oxygen consumption in a water-jacketed glass vessel (requires 150 islets)."
The Core "provides in depth consultation and helps develop strategies how to use the services of the core optimally or will attempt to modify available technologies to solve particular problems."
"Quantitating oxygen consumption of perifused freshly isolated or cultured islet tissue using optical methods (requires 500-600 islets)."
"Islet perfusion to measure insulin, glucagon and cAMP release (in connection with the Radioimmunoassay Core). Batch incubation experiments to measure insulin, glucagon and cAMP release (in connection with the Radioimmunoassay Core)."
Respiration and glycolysis measured by Seahorse System.