The mission of the Pathology Clinical Service Center (PCSC) is to promote and facilitate translational research by providing comprehensive blood and tissue-based services to investigators. Among these services are those that traditionally are only provided by Anatomic and Clinical Pathologists in the clinical setting. The Anatomic Division (PCSC-AP) specializes in the analysis of human bio-samples and offers histology, immunohistochemistry, immunofluorescence, in situ hybridization, tissue microarray construction, molecular analysis, digital imaging, multispectral image analysis and assay development. The Transfusion Medicine & Therapeutic Pathology Division (PSCS-TM&TP) encompasses the Apheresis and Infusion Clinic, the Stem Cell Lab, and the Blood Bank, and the Penn Medicine Blood Donation Center. The PCSC-TM&TP operates in compliance with FDA regulations, is accredited by the AABB and FACT, and specializes in the collection, processing, and re-infusion of cellular products, and offering mononuclear cell, whole blood, and plasma collections, elutriation, and infusion of intravenous medications under medical supervision. The Clinical Pathology Division (PCSC-CP) specializes in the analysis of blood and serum samples, including chemistry, microbiology, coagulation, hematology, immunology, and molecular pathology. In addition, PCSC can collect, store, analyze, and annotate research samples for IRB-approved projects.
"The TRIO™ system can capture up to three chromogens or up to three fluorophores simultaneously in a single tissue sample - delivering superior performance over filter-based systems. TRIO also offers multi-labeled per-cell measures of nuclear, cytoplasmic, and membranous protein expression, making it ideal for monitoring protein signaling pathways and resolving protein co-expression. The system is as straightforward to use as a point-and-shoot camera - advanced knowledge of multispectral imaging is not needed."
Clinical laboratory and pathology services are available through the Department of Pathology and Laboratory Medicine. Submit questions and and draft proposals to the director of Clinical Pathology Dr. Irving Nachamkin.
"The TDSC can help with project planning, tissue selection and experimental design. We can share antibody-specific protocols and provide technical assistance for those doing IHC in their own labs."
"The TDSC provides basic histology services including processing tissues and cell pellets, embedding, and cutting both frozen and paraffin-embedded samples. We can provide unstained slides, rolls, H&E's and some special stains."
"inForm™ advanced image analysis software has the ability to analyze only user-selected regions provides a powerful solution for performing nuclear, cytoplasmic, and membrane measurements on individual cells."
"The TDSC performs whole slide imaging using an Aperio ScanScope. Slides can be scanned using 20x or 40x objectives. The digital images are zoomable, can be stored on an external drive, and can be shared with others on the HUP network. The TDSC also offers users instruments capable of advanced microscopic image acquisition & analysis. The Vectra and TRIO multispectral slide imaging systems are available for multi-analyte detection and quantification in a single slide. The inForm analysis software allows users to train the system to analyze large numbers of slides."
"The TDSC performs both single and multiplex immunohistochemistry and immunofluorescence. We have an extensive menu of routinely used antibodies and provide a workup service for new antibodies. We have experience with multiple techniques and chromagens allowing us to optimize for the best results.
The TDSC has a Leica Bond III autostainer. This instrument enables us to run large studies with minimal variability and workup new tests for the clinical lab. The TDSC works closely with the clinical IHC lab and its Medical Director to ensure that the best clones and most up-to-date assays are in use."
"The TDSC cuts tissue for molecular analysis using a special clean-cutting protocol. We can macro-dissect tissue and perform nucleic acid extractions. We have a nanodrop, Bioanalyzer and qPCR machine available for QC and quantification. The TDSC can assist in developing new assays and has worked with the Molecular Diagnostic Lab."
"The Vectra™ system accurately measures protein expressions and morphometric characteristics on whole slides or in distinct tissue regions or TMAs. Imaged tissue sections can be labeled with immunofluorescent (IF) or immunohistochemical (IHC) stains, or with conventional stains such as H&E and trichrome. When using IF or IHC stains, one or multiple proteins can be measured on a per tissue, per cell, or per cell compartment (e.g. nuclear, cytoplasmic) basis - even when signals are spectrally similar, in the same cell compartment or obscured by autofluorescence."
"The TDSC can construct high and low-density arrays using 0.6, 1.0, 1.5 and 2.0 mm core sizes. The arrays are customized for your needs and can be optimized for multi-spectral image analysis.
To initiate a new TMA project, please contact the TMA Lab Director:"
"Training is required to use the Vectra multi-spectral imaging system and analysis software."
"Cell Analysis can phenotype cells based on their biomarker expression within cells, nuclei and membranes. It is designed for individual research groups or small tissue pathology studies.
• Enables tissues to be studied at the cellular level using the Cell/Object Segmentation functionality
• Enhanced tools for more accurate cell segmentation
• Visualizes, analyzes, quantifies and phenotypes immune and other cells in-situ in solid tissue
• Pathology Views(tm) to render immunofluorescence images as H&E, DAB and hematoxylin
• Automated quantitation of biomarker expression from specific tissue types (e.g. tumor/stroma)
• Separates weakly expressing and overlapping markers
• Batch processing of images using customizable image analysis workflows
• Scoring (% positivity, 0/1+/2+/3+, co-localization and more)
• Export data and images for further analysis, for example: TIBCO
• Spotfire® Analyst for Quantitative Pathology"